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rdf:type |
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lifeskim:mentions |
umls-concept:C0040648,
umls-concept:C0085358,
umls-concept:C0442805,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1419772,
umls-concept:C1513032,
umls-concept:C1514559,
umls-concept:C1706438,
umls-concept:C1709707,
umls-concept:C1881379,
umls-concept:C2698600
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pubmed:issue |
5
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pubmed:dateCreated |
2005-2-24
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pubmed:abstractText |
The Runx family of transcription factors is thought to regulate the differentiation of thymocytes. Runx3 protein is detected mainly in the CD4(-)8(+) subset of T lymphocytes. In the thymus of Runx3-deficient mice, CD4 expression is de-repressed and CD4(-)8(+) thymocytes do not develop. This clearly implicates Runx3 in CD4 silencing, but does not necessarily prove its role in the differentiation of CD4(-)8(+) thymocytes per se. In the present study, we created transgenic mice that overexpress Runx3 and analyzed the development of thymocytes in these animals. In the Runx3-transgenic thymus, the number of CD4(-)8(+) cells was greatly increased, whereas the numbers of CD4(+)8(+) and CD4(+)8(-) cells were reduced. The CD4(-)8(+) transgenic thymocytes contained mature cells with a TCR(high)HSA(low) phenotype. These cells were released from the thymus and contributed to the elevated level of CD4(-)8(+) cells relative to CD4(+)8(-) cells in the spleen. Runx3 overexpression also increased the number of mature CD4(-)8(+) thymocytes in mice with class II-restricted, transgenic TCR and in mice with a class I-deficient background, both of which are favorable for CD4(+)8(-) lineage selection. Thus, Runx3 can drive thymocytes to select the CD4(-)8(+) lineage. This activity is likely to be due to more than a simple silencing of CD4 gene expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1767
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pubmed:author |
pubmed-author:AbeNatsumiN,
pubmed-author:HabuSonokoS,
pubmed-author:HayashiKeitaroK,
pubmed-author:InoueYoshihiroY,
pubmed-author:IwakuraYoichiroY,
pubmed-author:KikuchiToshiakiT,
pubmed-author:KohuKazuyoshiK,
pubmed-author:NakazatoMegumiM,
pubmed-author:NaomiYoshidaY,
pubmed-author:OhnoShin-IchiroS,
pubmed-author:SatakeMasanobuM,
pubmed-author:SatoTakehitoT,
pubmed-author:UchinoRyujiR,
pubmed-author:WatanabeToshioT
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
174
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2627-36
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15728469-Animals,
pubmed-meshheading:15728469-Antigens, CD4,
pubmed-meshheading:15728469-Antigens, CD8,
pubmed-meshheading:15728469-CD4-Positive T-Lymphocytes,
pubmed-meshheading:15728469-CD8-Positive T-Lymphocytes,
pubmed-meshheading:15728469-Cell Differentiation,
pubmed-meshheading:15728469-Cell Lineage,
pubmed-meshheading:15728469-Cell Proliferation,
pubmed-meshheading:15728469-Core Binding Factor Alpha 3 Subunit,
pubmed-meshheading:15728469-DNA-Binding Proteins,
pubmed-meshheading:15728469-Gene Expression Profiling,
pubmed-meshheading:15728469-Lymphocyte Count,
pubmed-meshheading:15728469-Mice,
pubmed-meshheading:15728469-Mice, Inbred C3H,
pubmed-meshheading:15728469-Mice, Inbred C57BL,
pubmed-meshheading:15728469-Mice, Transgenic,
pubmed-meshheading:15728469-Signal Transduction,
pubmed-meshheading:15728469-T-Lymphocyte Subsets,
pubmed-meshheading:15728469-Thymus Gland,
pubmed-meshheading:15728469-Transcription Factors
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pubmed:year |
2005
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pubmed:articleTitle |
Overexpression of the Runx3 transcription factor increases the proportion of mature thymocytes of the CD8 single-positive lineage.
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pubmed:affiliation |
Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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