Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1992-5-28
pubmed:abstractText
The extracellular and intracellular domains of the human thyrotropin receptor were expressed in Escherichia coli and the proteins were used to produce monoclonal anti-receptor antibodies. Immunoblot studies and immunoaffinity purification showed that the receptor is composed of two subunits linked by disulfide bridges and probably derived by proteolytic cleavage of a single 90-kDa precursor. The extracellular alpha subunit (hormone binding) had an apparent molecular mass of 53 kDa (35 kDa after deglycosylation with N-glycosidase F). The membrane-spanning beta subunit seemed heterogeneous and had an apparent molecular mass of 33-42 kDa. Human thyroid membranes contained a 2.5- to 3-fold excess of beta subunits over alpha subunits. Immunocytochemistry showed the presence of both subunits in all the follicular thyroid cells, and both subunits were restricted to the basolateral region of the cell membrane.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-169249, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-1905715, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-1992482, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-1999172, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2070411, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2226303, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2293030, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2302212, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2453350, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2460448, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2471709, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2502844, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2537304, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2558651, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2610690, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2859121, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2983222, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-2988500, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-3004903, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-3117522, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-3286231, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-3533623, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-3665836, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-3678141, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-37068, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-567561, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-6088581, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-6098307, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-6298540, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-6315402, http://linkedlifedata.com/resource/pubmed/commentcorrection/1570295-6579531
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3765-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Two-subunit structure of the human thyrotropin receptor.
pubmed:affiliation
Institut National de la Santé et de la Recherche Médicale, Hôpital de Bicêtre, Le Kremlin Bicêtre, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't