Linked Life Data

15678478

Source:http://linkedlifedata.com/resource/pubmed/id/15678478

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-2-3
pubmed:abstractText
The birth and differentiation of neurons have been extensively studied in the olfactory epithelium (OE) of rodents but not in humans. The goal of this study was to characterize cellular composition and molecular expression of human OE in vivo and in vitro. In rodent OE, there are horizontal basal cells and globose basal cells that are morphologically and functionally distinct. In human OE, however, there appears to be no morphological distinction among basal cells, with almost all cells having round cell bodies similar to rodent globose basal cells. Unlike the case in rodents, human basal cells, including putative neuronal precursors, express p75NGFR, suggesting a distinctive role for p75NGFR in human OE neurogenesis. Molecular expression of neuronal cells during differentiation in human OE grossly follows that in rodents. However, the topographical organization of immature and mature ORNs in human OE differs from that of rodents, in that immature and mature ORNs in humans are dispersed throughout the OE, whereas rodent counterparts have a highly laminar organization. These observations together suggest that the birth and differentiation of neuronal cells in human OE differ from those in rodents. In OE explant culture, neuronal cells derived from human OE biopsy express markers for immature and mature neurons, grossly recapitulating neuronal differentiation of olfactory neurons in vivo. Furthermore, small numbers of cells are doubly label for bromodeoxyuridine and olfactory marker protein, indicating that neuronal cells born in vitro reach maturity. These data highlight species-related differences in OE development and demonstrate the utility of explant culture for experimental studies of human neuronal development.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9967
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
483
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
154-63
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15678478-Adult, pubmed-meshheading:15678478-Aged, pubmed-meshheading:15678478-Aged, 80 and over, pubmed-meshheading:15678478-Aging, pubmed-meshheading:15678478-Antigens, Differentiation, pubmed-meshheading:15678478-Bromodeoxyuridine, pubmed-meshheading:15678478-Cell Differentiation, pubmed-meshheading:15678478-Cell Proliferation, pubmed-meshheading:15678478-Cell Shape, pubmed-meshheading:15678478-Cells, Cultured, pubmed-meshheading:15678478-Humans, pubmed-meshheading:15678478-Immunohistochemistry, pubmed-meshheading:15678478-Middle Aged, pubmed-meshheading:15678478-Nerve Tissue Proteins, pubmed-meshheading:15678478-Neurons, pubmed-meshheading:15678478-Olfactory Marker Protein, pubmed-meshheading:15678478-Olfactory Mucosa, pubmed-meshheading:15678478-Receptor, Nerve Growth Factor, pubmed-meshheading:15678478-Receptors, Nerve Growth Factor, pubmed-meshheading:15678478-Stem Cells
pubmed:year
2005
pubmed:articleTitle
In vivo and in vitro neurogenesis in human olfactory epithelium.
pubmed:affiliation
Department of Psychiatry, University of Pennsylvania, Philadelphia, PA 19104, USA. hahnc@mail.med.upenn.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't