Source:http://linkedlifedata.com/resource/pubmed/id/15659611
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2005-1-20
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| pubmed:abstractText |
We have purified and characterized a novel conotoxin from the venom of Conus obscurus, which has the unique property of selectively and potently inhibiting the fetal form of the mammalian neuromuscular nicotinic acetylcholine receptor (nAChR) (alpha1beta1gammadelta-subunits). Although this conotoxin, alphaA-conotoxin OIVB (alphaA-OIVB), is a high-affinity antagonist (IC50 of 56 nm) of the fetal muscle nAChR, it has >1800-fold lower affinity for the adult muscle nAChR (alpha1beta1epsilondelta-subunits) and virtually no inhibitory activity at a high concentration on various neuronal nAChRs (IC50 > 100 microm in all cases). The peptide (amino acid sequence, CCGVONAACPOCVCNKTCG), with three disulfide bonds, has been chemically synthesized in a biologically active form. Although the neuromuscular nAChRs are perhaps the most extensively characterized of the receptors/ion channels of the nervous system, the precise physiological roles of the fetal form of the muscle nAChR are essentially unknown.alphaA-OIVB is a potentially important tool for delineating the functional roles ofalpha1beta1gammadelta receptors in normal development, as well as in various adult tissues and in pathological states. In addition to its potential as a research tool, alphaA-OIVB may have some direct biomedical applications.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Conotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Nicotinic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nicotinic,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1529-2401
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
19
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| pubmed:volume |
25
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
732-6
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:15659611-Adult,
pubmed-meshheading:15659611-Age Factors,
pubmed-meshheading:15659611-Amino Acid Sequence,
pubmed-meshheading:15659611-Animals,
pubmed-meshheading:15659611-Behavior, Animal,
pubmed-meshheading:15659611-Conotoxins,
pubmed-meshheading:15659611-Fetus,
pubmed-meshheading:15659611-Goldfish,
pubmed-meshheading:15659611-Humans,
pubmed-meshheading:15659611-Ligands,
pubmed-meshheading:15659611-Mice,
pubmed-meshheading:15659611-Molecular Sequence Data,
pubmed-meshheading:15659611-Muscles,
pubmed-meshheading:15659611-Neuromuscular Junction,
pubmed-meshheading:15659611-Nicotinic Antagonists,
pubmed-meshheading:15659611-Paralysis,
pubmed-meshheading:15659611-Patch-Clamp Techniques,
pubmed-meshheading:15659611-Receptors, Nicotinic,
pubmed-meshheading:15659611-Recombinant Proteins,
pubmed-meshheading:15659611-Snails,
pubmed-meshheading:15659611-Xenopus
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| pubmed:year |
2005
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| pubmed:articleTitle |
A uniquely selective inhibitor of the mammalian fetal neuromuscular nicotinic acetylcholine receptor.
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| pubmed:affiliation |
Department of Biology, University of Utah, Salt lake City, Utah 84112, USA. teichert@biology.utah.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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