Linked Life Data

15644119

Source:http://linkedlifedata.com/resource/pubmed/id/15644119

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-1-12
pubmed:abstractText
In rheumatoid arthritis (RA), fibroblasts have been shown to be crucial for disease progression as well as joint destruction. In the model of human/murine SCID arthritis, synovial explants as well as fibroblasts from human rheumatoid synovial membrane induce destructive arthritis in immunodeficient mice. Hereby, the underlying cartilage destruction is accomplished by murine fibroblasts. Therefore, murine destructive fibroblasts represent a promising tool to investigate destruction of articular cartilage and bone. In this context, a novel destructive murine fibroblast line (LS48) was examined for morphological, ultrastructural, immunological and functional cellular parameters. These cells were injected into knees of SCID mice. Subsequently, the animals were monitored for joint swelling and serological parameters of arthritis by radiological methods. Finally, cartilage destruction was assessed morphologically. Cultured LS48 cells exhibit characteristic features that resemble those of activated synovial fibroblasts in human RA. Expression levels of inducible nitric oxide synthase, interleukin-6, tumour necrosis factor-alpha and matrix metalloproteinases were comparable to those detected in invasive human fibroblasts. The instillation of 5 x 10(5) LS48 cells into the knee joints of SCID mice initiated a rapid progressive process, that caused cartilage destruction within 10 days, and morphological examinations revealed that articular cartilage was infiltrated by the fibroblasts injected previously. In summary, the intra-articular application of LS48 cells represents a rapid and highly reproducible model to investigate the initiation and progression of cartilage destruction in connection with RA therapy and represents an easy-to-handle animal model.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0300-9475
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18-28
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15644119-Animals, pubmed-meshheading:15644119-Arthritis, Rheumatoid, pubmed-meshheading:15644119-Cartilage, pubmed-meshheading:15644119-Cell Line, pubmed-meshheading:15644119-Coculture Techniques, pubmed-meshheading:15644119-Collagenases, pubmed-meshheading:15644119-Disease Models, Animal, pubmed-meshheading:15644119-Fibroblasts, pubmed-meshheading:15644119-Humans, pubmed-meshheading:15644119-Interleukin-6, pubmed-meshheading:15644119-Matrix Metalloproteinase 13, pubmed-meshheading:15644119-Matrix Metalloproteinase 2, pubmed-meshheading:15644119-Matrix Metalloproteinase 9, pubmed-meshheading:15644119-Mice, pubmed-meshheading:15644119-Mice, SCID, pubmed-meshheading:15644119-Microscopy, Electron, pubmed-meshheading:15644119-RNA, Messenger, pubmed-meshheading:15644119-Synovial Membrane
pubmed:year
2005
pubmed:articleTitle
A novel model of fibroblast-mediated cartilage destruction.
pubmed:affiliation
Institute of Clinical Immunology and Transfusion Medicine, University of Leipzig, Leipzig, Germany. ulrich.sack@medizin.uni-leipzig.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't