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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
1992-5-12
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L01675,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L01676,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L01677,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M68903,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M81836,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M83679,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M83680,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M83681,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M83724,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S75381
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pubmed:abstractText |
The complete analysis of a cDNA clone encoding the mouse integrin beta 7 subunit that was isolated from a lambda gt10 cDNA library prepared from interleukin-4-activated mouse spleen B cells is reported. The 805-amino acid sequence deduced from the cDNA revealed a signal peptide, a 704-amino acid extracellular domain with eight N-glycosylation sites, a transmembrane domain, and a 61-amino acid intracellular domain. Several structural features were conserved with other integrin beta chains including the four cysteine-rich epidermal growth factor-like repeat sequences in the extracellular domain. Comparative analysis of the mouse and human beta 7 subunits revealed 87% sequence identity with conservation of three potential intracellular tyrosine phosphorylation sites. The cDNA hybridized to two mRNA transcripts of 3 and 2 kilobases (kb) in size. The 3-kb transcript, which is considered the productive form, was found in T and B leukemic cell lines, a macrophage line, and a mastocytoma cell line. Suprisingly, the smaller 2-kb transcript, which seems unlikely to encode a complete beta 7 protein, was found expressed in epithelial cells cultured from mouse skin. The integrin beta 7 subunit displays sequence identity with the beta subunit of the M290 antigen found expressed almost exclusively on intraepithelial lymphocytes in the small intestine suggesting that beta 7 may play an adhesive role in intraepithelial lymphocytes immunosurveillance of the gut mucosa. The cellular distribution of beta 7 transcripts is more extensive than the T-lineage-restricted distribution of the M290 antigen, indicating that beta 7 may be the common beta subunit for more than one receptor or that translation of beta 7 transcripts varies in different cell types.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
|
pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
|
pubmed:volume |
267
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
7352-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1559978-Amino Acid Sequence,
pubmed-meshheading:1559978-Animals,
pubmed-meshheading:1559978-Base Sequence,
pubmed-meshheading:1559978-Blotting, Northern,
pubmed-meshheading:1559978-Cell Line,
pubmed-meshheading:1559978-Cloning, Molecular,
pubmed-meshheading:1559978-DNA,
pubmed-meshheading:1559978-Glycosylation,
pubmed-meshheading:1559978-Humans,
pubmed-meshheading:1559978-Integrins,
pubmed-meshheading:1559978-Mice,
pubmed-meshheading:1559978-Molecular Sequence Data,
pubmed-meshheading:1559978-Nucleic Acid Hybridization,
pubmed-meshheading:1559978-Phosphorylation,
pubmed-meshheading:1559978-Protein Sorting Signals,
pubmed-meshheading:1559978-RNA, Messenger,
pubmed-meshheading:1559978-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1559978-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
Molecular cloning of the mouse integrin beta 7 subunit.
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pubmed:affiliation |
Department of Molecular Medicine, School of Medicine, University of Auckland, New Zealand.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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