Linked Life Data

1559548

Source:http://linkedlifedata.com/resource/pubmed/id/1559548

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-5-13
pubmed:abstractText
Growth of the ocular lens is directed by the division and differentiation of a single layer of epithelial cells located at the equatorial region. It is conceivable that this region of the lens capsule presents a special microenvironment modulated by molecular cues emanating from the surrounding tissues. In an effort to investigate the source and nature of these molecular cues, we co-cultured human fetal lens epithelial cells and fibroblasts derived from the ciliary body. We observed morphological differentiation as evidenced by the appearance of differentiating lentoid structures associated with fibroblasts. Characterization of the expression of lens-specific proteins revealed that in addition to alpha B-crystallin, these lentoid structures contain the lens fiber cell-specific proteins, alpha A-crystallin, beta B2-crystallin and gamma S-crystallin. None of these crystallins could be found in the surrounding undifferentiated lens epithelial cells. Interestingly, alpha B-crystallin usually present in lens epithelial cells when cultured alone, was found to be markedly decreased, both in synthesis and content in the cells surrounding the differentiated structures, suggesting that the process of differentiation in vitro may concomitantly produce a factor(s) which modulates alpha B-crystallin expression in these cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0014-4835
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
193-200
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Lens fiber cell differentiation and expression of crystallins in co-cultures of human fetal lens epithelial cells and fibroblasts.
pubmed:affiliation
Jules Stein Eye Institute, University of California, School of Medicine, Los Angeles 90024-1771.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't