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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3 Pt 2
|
| pubmed:dateCreated |
1992-5-4
|
| pubmed:abstractText |
Metabolic acidosis induces net calcium flux (JCa) from cultured neonatal mouse calvariae through physicochemical and cell-mediated mechanisms. To determine the role of osteoblasts in acid-induced JCa, collagen synthesis and alkaline phosphatase activity were assessed in calvariae incubated in reduced pH and bicarbonate medium, a model of metabolic acidosis (Met), and compared with controls (Ctl). Collagen synthesis fell from 30.5 +/- 1.1 in Ctl to 25.1 +/- 0.4% with Met, and alkaline phosphatase decreased from 403 +/- 25 in Ctl to 298 +/- 21 nmol Pi.min-1.mg protein-1 with Met. During acidosis JCa was correlated inversely with percent collagen synthesis (r = -0.743, n = 11, P = 0.009) and with alkaline phosphatase activity (r = -0.453, n = 22, P = 0.034). To determine the role of osteoclasts in acid-induced JCa, osteoclastic beta-glucuronidase activity was determined in Ctl and Met in the absence or presence of the osteoclastic inhibitor calcitonin (CT, 3 x 10(-9) M). Met increased beta-glucuronidase (5.9 +/- 0.2) compared with Ctl (4.6 +/- 0.3 micrograms phenolphthalein released.bone-1.h-1), whereas CT inhibited beta-glucuronidase in both Ctl and Met (3.1 +/- 0.2 and 3.5 +/- 0.3, respectively). During acidosis JCa was correlated directly with beta-glucuronidase activity (r = 0.683, n = 42, P less than 0.001). Thus the cell-mediated component of JCa during acidosis in vitro appears to result from a combination of inhibited osteoblastic and stimulated osteoclastic activity.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
262
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
F442-8
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1558161-Acidosis,
pubmed-meshheading:1558161-Alkaline Phosphatase,
pubmed-meshheading:1558161-Animals,
pubmed-meshheading:1558161-Animals, Newborn,
pubmed-meshheading:1558161-Cells, Cultured,
pubmed-meshheading:1558161-Collagen,
pubmed-meshheading:1558161-Glucuronidase,
pubmed-meshheading:1558161-Hydrogen-Ion Concentration,
pubmed-meshheading:1558161-Kinetics,
pubmed-meshheading:1558161-Mice,
pubmed-meshheading:1558161-Mice, Inbred Strains,
pubmed-meshheading:1558161-Organ Culture Techniques,
pubmed-meshheading:1558161-Osteoblasts,
pubmed-meshheading:1558161-Osteoclasts,
pubmed-meshheading:1558161-Parathyroid Hormone,
pubmed-meshheading:1558161-Regression Analysis
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Acidosis inhibits osteoblastic and stimulates osteoclastic activity in vitro.
|
| pubmed:affiliation |
Department of Medicine, University of Rochester School of Medicine and Dentistry, New York 14642.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|