|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0017262,
umls-concept:C0023884,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0038250,
umls-concept:C0085110,
umls-concept:C0086418,
umls-concept:C0304925,
umls-concept:C0439659,
umls-concept:C1171362,
umls-concept:C1515655,
umls-concept:C1515670,
umls-concept:C1533691
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
2004-12-6
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|
pubmed:abstractText |
Studies in rodents suggest the presence of a hepatopancreatic stem cell in adult pancreas that may give rise to liver cells in vivo. The aim of the present study was to determine the ability of human islet-derived cells to adopt a hepatic phenotype in vivo. Cultured human islet-derived progenitor cells that did not express albumin in vitro were stained with the red fluorescent dye PKH26 and injected into the liver of severe combined immunodeficiency mice. After 3 or 12 weeks, red fluorescent cells were detected in 11 of 15 livers and were mostly single cells that were well integrated into the liver tissue. Human albumin was found in 8 of 11 animals by immunohistochemistry, and human albumin mRNA was detected in 4 of 10 host livers. The mechanism underlying this phenomenon seems to be transdifferentiation, because human and mouse albumin were found to be expressed in distinct cells in the host liver.
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
1066-5099
|
|
pubmed:author |
pubmed-author:BrulportMarcM,
pubmed-author:EberhardtMichaelM,
pubmed-author:GroscheJensJ,
pubmed-author:HengstlerJan GeorgJG,
pubmed-author:HermesMatthiasM,
pubmed-author:MüllerBeatB,
pubmed-author:PrawittDirkD,
pubmed-author:ReichenbachAndreasA,
pubmed-author:SchormannWiebkeW,
pubmed-author:WeilemannLudwig SachaLS,
pubmed-author:ZabelBernhardB,
pubmed-author:ZulewskiHenrykH,
pubmed-author:von MachMarc-AlexanderMA
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|
pubmed:issnType |
Print
|
|
pubmed:volume |
22
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1134-41
|
|
pubmed:dateRevised |
2006-11-15
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|
pubmed:meshHeading |
pubmed-meshheading:15579634-Albumins,
pubmed-meshheading:15579634-Animals,
pubmed-meshheading:15579634-Cell Differentiation,
pubmed-meshheading:15579634-Cell Transplantation,
pubmed-meshheading:15579634-Cells, Cultured,
pubmed-meshheading:15579634-Chromosome Banding,
pubmed-meshheading:15579634-Fluorescent Dyes,
pubmed-meshheading:15579634-Humans,
pubmed-meshheading:15579634-Immunohistochemistry,
pubmed-meshheading:15579634-Islets of Langerhans,
pubmed-meshheading:15579634-Karyotyping,
pubmed-meshheading:15579634-Liver,
pubmed-meshheading:15579634-Mice,
pubmed-meshheading:15579634-Mice, SCID,
pubmed-meshheading:15579634-Microscopy, Fluorescence,
pubmed-meshheading:15579634-Organic Chemicals,
pubmed-meshheading:15579634-Phenotype,
pubmed-meshheading:15579634-RNA, Messenger,
pubmed-meshheading:15579634-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15579634-Stem Cells,
pubmed-meshheading:15579634-Time Factors,
pubmed-meshheading:15579634-Transplantation, Heterologous
|
|
pubmed:year |
2004
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|
pubmed:articleTitle |
In vitro cultured islet-derived progenitor cells of human origin express human albumin in severe combined immunodeficiency mouse liver in vivo.
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|
pubmed:affiliation |
II. Medical Department, University of Mainz, Mainz, Germany. marcm@giftinfo.uni-mainz.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
