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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1992-5-4
|
| pubmed:abstractText |
We have evaluated a simple and sensitive culture technique for isolation of Human Immunodeficiency Virus Type-1 (HIV-1) from small amounts of whole blood. Data shown in the paper demonstrate that: 1) cell cultures from small amounts of heparinized whole blood (HWB) allow a high isolation rate in infected subjects at all stages of diseases; 2) among asymptomatic subjects the HIV-1 isolation rate is increased in cell cultures from HWB, with respect to cell cultures from peripheral blood mononuclear cells; 3) cultural results from HWB are not influenced by the presence of detectable serum p24 antigen, but a good correlation was found with the titre of anti p24 antibodies in serum; 4) continuous cell lines (such as Molt-3 cells) instead of peripheral blood mononuclear cells can be used, obtaining good results, for HIV-1 isolation from HWB; 5) frozen samples of HWB can be used in cell cultures for HIV-1 isolation; 6) the type of anticoagulant (Heparin or EDTA) used for the collection of blood does not influence viral replication in cell cultures from whole blood; 7) viral isolation from HWB is highly sensitive; amounts so small as five microliters of whole blood are sufficient, in some cases, to obtain viral replication in cell cultures; 8) the minimal dose of HWB sufficient to infect cell cultures (HWB M.D.I.) varied among different patients. Although this work failed to establish a correlation between this parameter and the clinical and immunological status of patients, it is conceivable that HWB M.D.I. could give information about viral load in blood and have a prognostic significance; 9) the HWB M.D.I. rise in patients treated with Zidovudine, suggesting that this method could be employed in the virological evaluation of trials with antiretroviral drugs.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Edetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Core Protein p24,
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Zidovudine
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0391-5352
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
15
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
35-44
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1556958-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:1556958-Cell Line,
pubmed-meshheading:1556958-Cells, Cultured,
pubmed-meshheading:1556958-Cryopreservation,
pubmed-meshheading:1556958-Edetic Acid,
pubmed-meshheading:1556958-Evaluation Studies as Topic,
pubmed-meshheading:1556958-HIV Antibodies,
pubmed-meshheading:1556958-HIV Core Protein p24,
pubmed-meshheading:1556958-HIV Infections,
pubmed-meshheading:1556958-HIV-1,
pubmed-meshheading:1556958-Heparin,
pubmed-meshheading:1556958-Humans,
pubmed-meshheading:1556958-Kinetics,
pubmed-meshheading:1556958-Leukocytes, Mononuclear,
pubmed-meshheading:1556958-Sensitivity and Specificity,
pubmed-meshheading:1556958-Time Factors,
pubmed-meshheading:1556958-Viremia,
pubmed-meshheading:1556958-Virus Replication,
pubmed-meshheading:1556958-Zidovudine
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
HIV-1 isolation from small amounts of whole blood: a technical evaluation.
|
| pubmed:affiliation |
Clinic of Infectious Diseases, University of Bari, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|