Linked Life Data

15562501

Source:http://linkedlifedata.com/resource/pubmed/id/15562501

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2004-12-27
pubmed:abstractText
Glutamine synthesis in the astroglia reflects the sum of neurotransmitter cycling (glutamate and gamma-aminobutyric acid [GABA]) and de novo synthesis (anaplerosis), the latter catalyzed by pyruvate carboxylase. Previous studies have shown that the glutamate plus GABA cycling flux is correlated strongly with neuronal activity; however, the relationship between pyruvate carboxylase flux and neuronal activity is not known. In this study, pyruvate carboxylase flux was assessed during intravenous infusion of [2-(13)C]glucose using localized (1)H-[(13)C] NMR spectroscopy at 7 Tesla in vivo in halothane-anesthetized and ventilated adult Wistar rats during 85 min of bicuculline-induced seizures (1 mg/kg, intravenously) and in nontreated controls. During seizures, concentrations of lactate, alanine, glutamine, GABA, and succinate increased whereas glutamate and aspartate decreased such that the decrease in glutamate plus aspartate equaled the increase in glutamine plus GABA. Pyruvate carboxylase flux was assessed by the sum of [2-(13)C] and [3-(13)C] of glutamine and glutamate (Glx(2+3)) labeling during [2-(13)C]glucose infusion. During seizures the initial rate of Glx(2+3) synthesis (0.069 +/- 0.013 micromol/g/min) was not significantly different (P = 0.68) from that of the controls (0.059 +/- 0.010 micromol/g/min), indicating that anaplerotic flow through pyruvate carboxylase was unaltered. Intense neuronal activation of seizures did not seem to increase anaplerosis through pyruvate carboxylase, despite the substantial increase in neuronal activity and glutamate/glutamine cycling shown in a previous study (Patel et al., 2004b).
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0360-4012
pubmed:author
pubmed:copyrightInfo
(c) 2004 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
128-38
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15562501-Amino Acids, pubmed-meshheading:15562501-Analysis of Variance, pubmed-meshheading:15562501-Animals, pubmed-meshheading:15562501-Bicuculline, pubmed-meshheading:15562501-Brain Chemistry, pubmed-meshheading:15562501-Carbon Isotopes, pubmed-meshheading:15562501-Cell Fractionation, pubmed-meshheading:15562501-Cell Membrane, pubmed-meshheading:15562501-Cerebellum, pubmed-meshheading:15562501-Citric Acid Cycle, pubmed-meshheading:15562501-Electroencephalography, pubmed-meshheading:15562501-Glucose, pubmed-meshheading:15562501-Magnetic Resonance Spectroscopy, pubmed-meshheading:15562501-Neurons, pubmed-meshheading:15562501-Probability, pubmed-meshheading:15562501-Pyruvate Carboxylase, pubmed-meshheading:15562501-Rats, pubmed-meshheading:15562501-Rats, Wistar, pubmed-meshheading:15562501-Seizures, pubmed-meshheading:15562501-Succinic Acid, pubmed-meshheading:15562501-Time Factors, pubmed-meshheading:15562501-gamma-Aminobutyric Acid
pubmed:articleTitle
Cerebral pyruvate carboxylase flux is unaltered during bicuculline-seizures.
pubmed:affiliation
Department of Diagnostic Radiology and Magnetic Resonance Research Center, Yale University School of Medicine, PO Box 208043, 300 Cedar Street, New Haven, CT 06520, USA. anant.patel@yale.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.