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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1992-5-7
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pubmed:abstractText |
Using random Tn10 insertion mutagenesis, we isolated an Escherichia coli mutant strain affected in the regulation of lysU, the gene encoding the inducible form of lysyl-tRNA synthetase. The transposon giving rise to the altered expression of lysU was found inserted within lrp. The latter gene codes for the leucine-responsive regulatory protein (Lrp) which mediates a global response of the bacterium to leucine. An involvement of Lrp in the regulation of lysU was searched for by using a lysU-lacZ operon fusion. The following conclusions were reached: (i) inactivation of lrp causes an increased activity of the lysU promoter, whatever the growth conditions assayed, (ii) insertion of a wild-type lrp gene into a multi-copy plasmid significantly reduces lysU expression, and (iii) sensitivity of the lysU promoter to the presence of leucine in the growth medium is abolished in the lrp context.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
300
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
254-8
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pubmed:dateRevised |
2006-5-1
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pubmed:meshHeading |
pubmed-meshheading:1555652-Bacterial Proteins,
pubmed-meshheading:1555652-Base Sequence,
pubmed-meshheading:1555652-Chromosome Mapping,
pubmed-meshheading:1555652-Chromosomes, Bacterial,
pubmed-meshheading:1555652-DNA-Binding Proteins,
pubmed-meshheading:1555652-Escherichia coli,
pubmed-meshheading:1555652-Escherichia coli Proteins,
pubmed-meshheading:1555652-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:1555652-Leucine,
pubmed-meshheading:1555652-Leucine-Responsive Regulatory Protein,
pubmed-meshheading:1555652-Lysine-tRNA Ligase,
pubmed-meshheading:1555652-Molecular Sequence Data,
pubmed-meshheading:1555652-Mutation,
pubmed-meshheading:1555652-Plasmids,
pubmed-meshheading:1555652-Transcription Factors
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pubmed:year |
1992
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pubmed:articleTitle |
Escherichia coli leucine-responsive regulatory protein (Lrp) controls lysyl-tRNA synthetase expression.
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pubmed:affiliation |
Laboratoire de Biochimie, Unité de Recherche Associée 240 du Centre National de la Recherche Scientifique, Ecole Polytechnique, Palaiseau, France.
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pubmed:publicationType |
Journal Article
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