Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-11-23
pubmed:abstractText
Androgen receptor (AR) recognizes and binds to 15-bp palindromic androgen response element (ARE) sequences with high affinity in vitro, which consist of two hexameric half-sites arranged as inverted repeats with a 3-bp spacer. Although a few near-consensus ARE sequences have been actually identified in the transcriptional regulatory regions of androgen-responsive genes, it has been unclear whether the exact consensus sequences function as bona fide AREs in vivo. A genome-wide in silico screening of palindromic AREs identified 563 exact consensus sequences in the human genome. The distribution of perfect palindromic AREs among the chromosomes is basically consistent with the length of chromosomes. Using human prostate cancer cell line LNCaP treated with a synthetic androgen R1881 as a model, in vivo AR binding abilities of 21 consensus AREs were analyzed by chromatin immunoprecipitation. Of 21 genomic fragments containing perfect AREs in chromosome X, 8 fragments recruited more ARs (>4-fold enrichment) even compared with the proximal ARE region of prostate-specific antigen. A couple of proximal genes or putative transcripts in the vicinity of the perfect AREs were found to be androgen-responsive analyzed by quantitative RT-PCR. Our results suggest that some of perfect palindromic AREs could function as in vivo AR binding sites in the human genome and regulate gene transcription.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
325
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1312-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Identification and functional analysis of consensus androgen response elements in human prostate cancer cells.
pubmed:affiliation
Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical School, 1397-1 Yamane, Hidaka-shi, Saitama 350-1241, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't