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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0011306,
umls-concept:C0205252,
umls-concept:C0205263,
umls-concept:C0871261,
umls-concept:C1515655,
umls-concept:C1533691,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1880022,
umls-concept:C2911692
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pubmed:issue |
4
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pubmed:dateCreated |
2004-11-23
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pubmed:abstractText |
Regulatory T (Treg) cells, derived from co-cultures of unfractionated CD4(+) T cells and immature dendritic cells (DC), suppress enteroantigen-induced proliferation of CD4(+) CD25(-) T cells. The DC-induced Treg cells are a mixture of CD25(+) (10-20%) and CD25(-) (80-90%) T cells. However, all the suppressor activity in vitro and in vivo resides in the CD25(+) T-cell subset. The CD25(+) DC-induced Treg cells can inhibit enteroantigen-induced proliferation in vitro through a transwell membrane, and their function does not appear to depend on previous activation. DC-induced CD25(+) Treg cells display a naive phenotype, expressing high levels of CD45RB and l-selectin (CD62L). In addition, the DC-induced Treg cells mediate a stronger suppressive activity than prototype CD25(+) regulatory T cells. The DC-induced Treg cells, and hereof purified CD25(+) and CD25(-) T-cell fractions, were co-injected into severe combined immunodeficiency (SCID) mice with colitis-inducing CD4(+) CD25(-) T cells. Both unfractionated CD4(+) and purified CD25(+) Treg cells fully protected the recipients against the development of colitis. In contrast, co-transfer of fractionated CD25(-) T cells offered no protection against disease development. Enterobacterial antigen-exposed CD4(+) T cells of the protected mice secreted higher levels of interleukin-10 and lower levels of interferon-gamma than the unprotected mice. The present data demonstrate DC-induced CD4(+) CD25(+) Treg cells, which phenotypically and functionally differ from the generally accepted prototype of CD25(+) Treg cells. These data may initiate new procedures for the expansion of Treg cells for clinical use.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-10228007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-10361228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-10510089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-10540183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-10795741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-10899916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-10964540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-11067871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-11106565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-11169434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-11247637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-11677084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-11722623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-12093005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-12119349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-12119350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-12202155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-12225384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-12444126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-12682220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-12727620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-12826082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-12874257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-12874259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-14991599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-1916494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-8100269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-8536356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-8607501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-8676081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-9120308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-9338786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-9500788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-9521319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-9588837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15554928-9784526
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
113
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
499-508
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15554928-Animals,
pubmed-meshheading:15554928-Antigens, Bacterial,
pubmed-meshheading:15554928-CD4-Positive T-Lymphocytes,
pubmed-meshheading:15554928-Cell Division,
pubmed-meshheading:15554928-Cells, Cultured,
pubmed-meshheading:15554928-Coculture Techniques,
pubmed-meshheading:15554928-Colitis,
pubmed-meshheading:15554928-Cytokines,
pubmed-meshheading:15554928-Dendritic Cells,
pubmed-meshheading:15554928-Female,
pubmed-meshheading:15554928-Immunophenotyping,
pubmed-meshheading:15554928-Interleukin-2,
pubmed-meshheading:15554928-Mice,
pubmed-meshheading:15554928-Mice, Inbred BALB C,
pubmed-meshheading:15554928-Mice, SCID,
pubmed-meshheading:15554928-Receptors, Interleukin-2,
pubmed-meshheading:15554928-T-Lymphocyte Subsets
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pubmed:year |
2004
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pubmed:articleTitle |
Characterization of T-regulatory cells, induced by immature dendritic cells, which inhibit enteroantigen-reactive colitis-inducing T-cell responses in vitro and in vivo.
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pubmed:affiliation |
Laboratory of Experimental Immunology, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, Copenhagen, Denmark. m.gad@mami.ku.dk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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