Linked Life Data

15548668

Source:http://linkedlifedata.com/resource/pubmed/id/15548668

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
46
pubmed:dateCreated
2004-11-19
pubmed:abstractText
We used a 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP)-enhanced green fluorescent protein (EGFP) transgenic mouse to study postnatal subventricular zone (SVZ) progenitor fate, with a focus on the olfactory bulb (OB). The postnatal OB of the CNP-EGFP mouse contained EGFP+ interneurons and oligodendrocytes. In the anterior SVZ, the majority of EGFP+ progenitors were NG2+. These NG2+/EGFP+ progenitors expressed the OB interneuron marker Er81, the neuroblast markers doublecortin (DC) and Distalless-related homeobox (DLX), or the oligodendrocyte progenitor marker Nkx2.2. In the rostral migratory stream (RMS), EGFP+ cells displayed a migrating phenotype. A fraction of these cells were either NG2-/Er81+/DC+/DLX+ or NG2+/Nkx2.2+. DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) injection into the lateral ventricle (LV) of early postnatal mice demonstrated that NG2+/EGFP+ progenitors migrate from the SVZ through the RMS into the OB. Moreover, fluorescence-activated cell-sorting-purified NG2+/CNP-EGFP+ or NG2+/beta-actin-enhanced yellow fluorescent protein-positive (EYFP+) progenitors transplanted into the early postnatal LV displayed extensive rostral and caudal migration. EYFP+ or EGFP+ graft-derived cells within the RMS were DLX+/Er81+ or Nkx2.2+, migrated to the OB, and differentiated to interneurons and oligodendrocytes. In the subcortical white matter (SCWM), grafted cells differentiated to either oligodendrocytes or astrocytes. Transplantation of NG2+/EYFP+ progenitors selectively purified from the SVZ showed that these cells were migratory and generated glia and neurons in the OB, hippocampus, and striatum. In contrast, cortical, OB, or cerebellar NG2+ cells had a very limited migratory potential and gave rise to glia in the SCWM and striatum. Our findings indicate region-specific differences between NG2+ progenitor cells and show that NG2+ cells can migrate throughout the RMS and contribute to both gliogenesis and neurogenesis in the postnatal OB.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
17
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10530-41
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15548668-Animals, pubmed-meshheading:15548668-Animals, Newborn, pubmed-meshheading:15548668-Antigens, pubmed-meshheading:15548668-Astrocytes, pubmed-meshheading:15548668-Brain, pubmed-meshheading:15548668-Cell Differentiation, pubmed-meshheading:15548668-Cell Movement, pubmed-meshheading:15548668-Cells, Cultured, pubmed-meshheading:15548668-Cerebral Ventricles, pubmed-meshheading:15548668-Green Fluorescent Proteins, pubmed-meshheading:15548668-Immunohistochemistry, pubmed-meshheading:15548668-Interneurons, pubmed-meshheading:15548668-Mice, pubmed-meshheading:15548668-Mice, Transgenic, pubmed-meshheading:15548668-Multipotent Stem Cells, pubmed-meshheading:15548668-Olfactory Bulb, pubmed-meshheading:15548668-Oligodendroglia, pubmed-meshheading:15548668-Proteoglycans, pubmed-meshheading:15548668-Stem Cell Transplantation, pubmed-meshheading:15548668-Stem Cells
pubmed:year
2004
pubmed:articleTitle
Postnatal neurogenesis and gliogenesis in the olfactory bulb from NG2-expressing progenitors of the subventricular zone.
pubmed:affiliation
Center for Neuroscience Research, Children's Research Institute, Children's National Medical Center, Washington, DC 20010, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural