|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0027651,
umls-concept:C0040223,
umls-concept:C0175631,
umls-concept:C0439851,
umls-concept:C0596795,
umls-concept:C0851827,
umls-concept:C1428114,
umls-concept:C1515655,
umls-concept:C1524066,
umls-concept:C1552596,
umls-concept:C1701901,
umls-concept:C1704711,
umls-concept:C1947931,
umls-concept:C2827662
|
|
pubmed:dateCreated |
2004-11-29
|
|
pubmed:abstractText |
Electroporation is currently receiving much attention as a way to increase drug and DNA delivery. Recent studies demonstrated the feasibility of electrogene therapy using a range of therapeutic genes for the treatment of experimental tumors. However, the transfection efficiency of electroporation-assisted DNA delivery is still low compared to viral methods and there is a clear need to optimize this approach. In order to optimize treatment, knowledge about spatial and time dependency of gene expression following delivery is of utmost importance in order to improve gene delivery. Intravital microscopy of tumors growing in dorsal skin fold window chambers is a useful method for monitoring gene transfection, since it allows non-invasive dynamic monitoring of gene expression in tumors in a live animal.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-10434041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-10504711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-11059581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-11198480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-11206915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-11221826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-11309280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-11350889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-11522635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-11818537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-11960291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-12001988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-12189527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-12516054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-12648161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-12972356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-14683587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-4667921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-8640760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15546484-9487524
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
1471-2407
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
16
|
|
pubmed:volume |
4
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
81
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:15546484-Animals,
pubmed-meshheading:15546484-Carcinosarcoma,
pubmed-meshheading:15546484-DNA,
pubmed-meshheading:15546484-Electroporation,
pubmed-meshheading:15546484-Gene Therapy,
pubmed-meshheading:15546484-Green Fluorescent Proteins,
pubmed-meshheading:15546484-Liposomes,
pubmed-meshheading:15546484-Male,
pubmed-meshheading:15546484-Microscopy,
pubmed-meshheading:15546484-Plasmids,
pubmed-meshheading:15546484-Rats,
pubmed-meshheading:15546484-Transfection
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
Direct visualization of electroporation-assisted in vivo gene delivery to tumors using intravital microscopy - spatial and time dependent distribution.
|
|
pubmed:affiliation |
Department of Experimental Oncology, Institute of Oncology, Zaloska 2, SI-1000 Ljubljana, Slovenia. mcemazar@onko-i.si
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
