Linked Life Data

15541310

Source:http://linkedlifedata.com/resource/pubmed/id/15541310

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-11-15
pubmed:abstractText
Inhibitors in myelin play a major role in preventing spontaneous axonal regeneration after CNS injury. Elevation of cAMP overcomes this inhibition, in a transcription-dependent manner, through the upregulation of Arginase I (Arg I) and increased synthesis of polyamines. Here, we show that the cAMP effect requires activation of the transcription factor cAMP response element binding protein (CREB) to overcome myelin inhibitors; a dominant-negative CREB abolishes the effect, and neurons expressing a constitutively active form of CREB are not inhibited. Activation of CREB is also required for cAMP to upregulate Arg I, and the ability of constitutively active CREB to overcome inhibition is blocked by an inhibitor of polyamine synthesis. Finally, expression of constitutively active CREB in DRG neurons is sufficient to promote regeneration of subsequently lesioned dorsal column axons. These results indicate that CREB plays a central role in overcoming myelin inhibitors and so encourages regeneration in vivo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0896-6273
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
609-21
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15541310-Animals, pubmed-meshheading:15541310-Arginase, pubmed-meshheading:15541310-Axons, pubmed-meshheading:15541310-Blotting, Western, pubmed-meshheading:15541310-Brain-Derived Neurotrophic Factor, pubmed-meshheading:15541310-Cerebellum, pubmed-meshheading:15541310-Cyclic AMP, pubmed-meshheading:15541310-Cyclic AMP Response Element-Binding Protein, pubmed-meshheading:15541310-Ganglia, Spinal, pubmed-meshheading:15541310-Immunohistochemistry, pubmed-meshheading:15541310-Male, pubmed-meshheading:15541310-Mice, pubmed-meshheading:15541310-Mice, Transgenic, pubmed-meshheading:15541310-Myelin Sheath, pubmed-meshheading:15541310-Myelin-Associated Glycoprotein, pubmed-meshheading:15541310-Nerve Regeneration, pubmed-meshheading:15541310-Rats, pubmed-meshheading:15541310-Rats, Long-Evans
pubmed:year
2004
pubmed:articleTitle
Activated CREB is sufficient to overcome inhibitors in myelin and promote spinal axon regeneration in vivo.
pubmed:affiliation
Biology Department, Hunter College, City University of New York, 695 Park Avenue, New York, NY 10021, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't