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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2004-10-26
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pubmed:abstractText |
Expression of the Wilms' tumor gene W T1 in primary astrocytic tumors was examined using a quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) or immunohistochemistry. Real-time RT-PCR showed that W T1 mRNA was expressed at various levels in all of the 25 astrocytic tumors examined. Immunohistochemical analysis showed that W T1 protein was expressed in 5 of 6 low-grade astrocytic tumors (grade I-II) and all of 18 high-grade ones (grade III-IV), and that expression levels of W T1 protein in high-grade tumors were significantly higher than those in low-grade ones. W T1 protein was not detected in the normal glial cells contained in the tumor specimens. Furthermore, treatment with W T1 antisense oligomers specifically inhibited growth of glioblastoma cell lines, U87-MG, A172, and T-98G. These results may indicate that the W T1 gene plays an important role in tumorigenesis of primary astrocytic tumors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1347-9032
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pubmed:author |
pubmed-author:AbenoSakieS,
pubmed-author:AoyagiSayakaS,
pubmed-author:AozasaKatsuyukiK,
pubmed-author:HosenNaokiN,
pubmed-author:JomgeowTanyaratT,
pubmed-author:KanatoKeisukeK,
pubmed-author:KawakamiManabuM,
pubmed-author:MarunoMotohikoM,
pubmed-author:NakanoYokoY,
pubmed-author:NakatsukaShin-IchiS,
pubmed-author:NakazawaTsutomuT,
pubmed-author:NishidaSumiyukiS,
pubmed-author:OjiYusukeY,
pubmed-author:OkaYoshihiroY,
pubmed-author:RoyV VVV,
pubmed-author:ShirakataToshiakiT,
pubmed-author:SugiyamaHaruoH,
pubmed-author:SuzukiTsuyoshiT,
pubmed-author:TatsumiNaoyaN,
pubmed-author:TsuboiAkihiroA,
pubmed-author:YokotaAsumiA,
pubmed-author:YoshimineToshikiT
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pubmed:issnType |
Print
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
822-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15504250-Adolescent,
pubmed-meshheading:15504250-Aged,
pubmed-meshheading:15504250-Astrocytoma,
pubmed-meshheading:15504250-Brain Neoplasms,
pubmed-meshheading:15504250-Cell Line, Tumor,
pubmed-meshheading:15504250-Cytoplasm,
pubmed-meshheading:15504250-Female,
pubmed-meshheading:15504250-Gene Expression,
pubmed-meshheading:15504250-Genes, Wilms Tumor,
pubmed-meshheading:15504250-Glioblastoma,
pubmed-meshheading:15504250-Humans,
pubmed-meshheading:15504250-Immunohistochemistry,
pubmed-meshheading:15504250-Male,
pubmed-meshheading:15504250-Middle Aged,
pubmed-meshheading:15504250-Neuroglia,
pubmed-meshheading:15504250-RNA, Messenger,
pubmed-meshheading:15504250-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15504250-Tumor Cells, Cultured,
pubmed-meshheading:15504250-WT1 Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
Overexpression of the Wilms' tumor gene W T1 in primary astrocytic tumors.
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pubmed:affiliation |
Department of Bioinformatics, Osaka University Graduate School of Medicine, 1-7 Yamada-Oka, Suita, Osaka 565-0871, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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