Linked Life Data

15494483

Source:http://linkedlifedata.com/resource/pubmed/id/15494483

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2004-10-20
pubmed:abstractText
In recent years, the quantification of T cell responses to pathogens or immunogens has become a common tool in the evaluation of disease pathogenesis or vaccine immunogenicity. Such measurements are usually limited to enumerating IFN-gamma-producing cells after ex vivo stimulation with Ag, but little is known about the phenotype or complete functional repertoire of the Ag-specific cells. We used 12-color flow cytometry to characterize Ag-specific T cells elicited by vaccines or natural infection to determine lineage and differentiation status as well as the capacity to produce four cytokines (IFN-gamma, TNF-alpha, IL-2, and IL-4) and a chemokine (MIP1beta). As expected, responding cells had a typical memory phenotype; however, the cytokine profiles associated with the responses were highly complex. The pattern of cytokine coexpression in response to specific Ags was a skewed subset of the complete repertoire (revealed by polyclonal stimulation). We found significant differences in the patterns of cytokines elicited by vaccination (where IFN-gamma was by far a subdominant response) vs natural infection; in addition, there was fairly significant intersubject variation. Our findings illustrate the limitation of the evaluation of immune responses using single functional measurements (such as IFN-gamma); in fact, it is likely that sensitive evaluation of Ag-specific T cells will require the coordinate measurement of several cytokines. The presence and variability of these complex response profiles introduce the possibility that selective functional expression patterns may provide correlates for vaccine efficacy or disease progression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
173
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5372-80
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15494483-AIDS Vaccines, pubmed-meshheading:15494483-CD4-Positive T-Lymphocytes, pubmed-meshheading:15494483-Chronic Disease, pubmed-meshheading:15494483-Cytokines, pubmed-meshheading:15494483-Epitopes, T-Lymphocyte, pubmed-meshheading:15494483-Flow Cytometry, pubmed-meshheading:15494483-HIV Infections, pubmed-meshheading:15494483-Hepatitis B Vaccines, pubmed-meshheading:15494483-Humans, pubmed-meshheading:15494483-Immunization, Secondary, pubmed-meshheading:15494483-Immunologic Memory, pubmed-meshheading:15494483-Immunophenotyping, pubmed-meshheading:15494483-Interferon-gamma, pubmed-meshheading:15494483-Interleukin-2, pubmed-meshheading:15494483-Lymphocyte Activation, pubmed-meshheading:15494483-T-Lymphocyte Subsets, pubmed-meshheading:15494483-T-Lymphocytes, Helper-Inducer, pubmed-meshheading:15494483-T-Lymphocytes, Regulatory, pubmed-meshheading:15494483-Tetanus Toxoid
pubmed:year
2004
pubmed:articleTitle
Vaccination in humans generates broad T cell cytokine responses.
pubmed:affiliation
Vaccine Research Center, National Institute of Allery and Infectious Diseases, NIH, Bethesda, MD 20892, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.