Linked Life Data

15492819

Source:http://linkedlifedata.com/resource/pubmed/id/15492819

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2004-10-19
pubmed:abstractText
In our previous investigations, mitogen-activated protein kinase kinase 2 (MEK2)/extracellular signal-regulated kinase 2 (ERK2) signaling pathway was found to be correlated with the cell dissociation induced by dissociation factor (DF) in pancreatic cancer cells. In this study, the expressions of epidermal growth factor receptor (EGFR), phosphorylated EGFR (p-EGFR), and its downstream kinases MEK1/2 and ERK1/2, were analyzed to clarify the regulatory mechanism of cell dissociation in pancreatic cancer cells. Two hamster (PC-1.0 and PC-1) and two human (AsPC-1 and Capan-2) pancreatic cancer cell lines were used. Immunocytochemical study was performed using anti-EGFR, p-EGFR, phosphorylated MEK1/2 (p-MEK1/2), and phosphorylated ERK1/2 (p-ERK1/2) antibodies. DF-treatment markedly induced the expressions of EGFR, p-EGFR, p-MEK1/2, p-ERK1/2, as well as the dissociation of cell colonies in PC-1 and Capan-2 cells. In contrast, AG1478 (an EGFR inhibitor) treatment significantly induced the cell aggregation in PC-1.0 and AsPC-1 cells which usually grew as single cells, but strongly suppressed the expressions of EGFR, p-EGFR, p-MEK1/2, and p-ERK1/2. These observations demonstrate that activation of EGFR is closely involved in cell dissociation in pancreatic cancer through activating MEK/ERK signaling pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1019-6439
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1303-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15492819-Adenocarcinoma, pubmed-meshheading:15492819-Animals, pubmed-meshheading:15492819-Cell Movement, pubmed-meshheading:15492819-Cricetinae, pubmed-meshheading:15492819-Immunohistochemistry, pubmed-meshheading:15492819-MAP Kinase Kinase 1, pubmed-meshheading:15492819-MAP Kinase Kinase 2, pubmed-meshheading:15492819-Mesocricetus, pubmed-meshheading:15492819-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:15492819-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:15492819-Neoplasm Invasiveness, pubmed-meshheading:15492819-Neoplasm Metastasis, pubmed-meshheading:15492819-Pancreatic Neoplasms, pubmed-meshheading:15492819-Phosphorylation, pubmed-meshheading:15492819-Receptor, Epidermal Growth Factor, pubmed-meshheading:15492819-Signal Transduction, pubmed-meshheading:15492819-Tumor Cells, Cultured
pubmed:year
2004
pubmed:articleTitle
Relationship between activation of epidermal growth factor receptor and cell dissociation in pancreatic cancer.
pubmed:affiliation
Department of Surgery II, Kumamoto University Medical School, Honjo 1-1-1, Kumamoto 860-8556, Japan. tanxd@hotmail.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't