Linked Life Data

15473668

Source:http://linkedlifedata.com/resource/pubmed/id/15473668

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2004-10-11
pubmed:abstractText
The development of an antibody labeling method with 99mTc is important for cancer imaging. Most bifunctional chelate methods for 99mTc labeling of antibody incorporate a 99mTc chelator through a linkage to lysine residue. In the present study, a novel site-specific 99mTc labeling method at carbohydrate side chain in the Fc region of 2 antibodies (T101 and rabbit anti-human serum albumin antibody (RPAb)) using dihydrazinophthalazine (DHZ) which has 2 hydrazino groups was developed. The antibodies were oxidized with sodium periodate to produce aldehyde on the Fc region. Then, one hydrazine group of DHZ was conjugated with an aldehyde group of antibody through the formation of a hydrazone. The other hydrazine group was used for labeling with 99mTc. The number of conjugated DHZ was 1.7 per antibody. 99mTc labeling efficiency was 46-85% for T101 and 67-87% for RPAb. Indirect labeling with DHZ conjugated antibodies showed higher stability than direct labeling with reduced antibodies. High immunoreactivities were conserved for both indirectly and directly labeled antibodies. A biodistribution study found high blood activity related to directly labeled T101 at early time point as well as low liver activity due to indirectly labeled T101 at later time point. However, these findings do not affect practical use. No significantly different biodistribution was observed in the other organs. The research concluded that DHZ can be used as a site-specific bifunctional chelating agent for labeling antibody with 99mTc. Moreover, 99mTc labeled antibody via DHZ was found to have excellent chemical and biological properties for nuclear medicine imaging.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0253-6269
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
961-7
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Site-specific 99mTc-labeling of antibody using dihydrazinophthalazine (DHZ) conjugation to Fc region of heavy chain.
pubmed:affiliation
Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul 110-744, Korea. jmjng@snu.ac.kr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't