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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
54
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pubmed:dateCreated |
2004-11-19
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pubmed:abstractText |
p21SNFT (21 kDa small nuclear factor isolated from T cells) is a human basic leucine zipper transcription factor that can repress AP-1-mediated transcription. We show here that overexpression of p21SNFT in HepG2 cells leads to repression of matrix metalloproteinase-1 by 70-80%. p21SNFT interacted with Jun at the matrix metalloproteinase-1 promoter -88 Ets/AP-1 enhancer element, where Jun is known to activate transcription via interaction with Fos and Ets proteins. When p21SNFT/Jun dimers bound the element in the presence of Ets, DNA was protected differently than when Fos was paired with Jun. The data suggest a difference in overall conformation between p21SNFT-containing and Fos-containing complexes that may be involved in the repression of matrix metalloproteinase-1 by p21SNFT. Overexpression of p21SNFT led to a reduction in invasiveness of HepG2 cells through type I collagen and reconstituted basement membrane, an effect similar to that obtained via direct immunodepletion of matrix metalloproteinase-1. The results indicate that the mechanism of repression of matrix metalloproteinase-1 by p21SNFT may be exploited in inhibiting pathological matrix remodeling during cancer progression in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8805-14
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15467742-Base Sequence,
pubmed-meshheading:15467742-Basic-Leucine Zipper Transcription Factors,
pubmed-meshheading:15467742-Carcinoma, Hepatocellular,
pubmed-meshheading:15467742-Cell Line, Tumor,
pubmed-meshheading:15467742-Collagen,
pubmed-meshheading:15467742-DNA Primers,
pubmed-meshheading:15467742-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:15467742-Enhancer Elements, Genetic,
pubmed-meshheading:15467742-Humans,
pubmed-meshheading:15467742-Immunoprecipitation,
pubmed-meshheading:15467742-Liver Neoplasms,
pubmed-meshheading:15467742-Matrix Metalloproteinase 1,
pubmed-meshheading:15467742-Neoplasm Invasiveness,
pubmed-meshheading:15467742-Promoter Regions, Genetic,
pubmed-meshheading:15467742-Repressor Proteins,
pubmed-meshheading:15467742-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15467742-Transcription, Genetic,
pubmed-meshheading:15467742-Transcription Factors
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pubmed:year |
2004
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pubmed:articleTitle |
Transcriptional repression of MMP-1 by p21SNFT and reduced in vitro invasiveness of hepatocarcinoma cells.
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pubmed:affiliation |
Department of Biology, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182-4614, USA. kbower@scripps.edu
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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