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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-10-6
pubmed:abstractText
The CDK inhibitor, p21, exhibits an antiapoptotic or proapoptotic effect, in addition to its anti-proliferative effect, depending on the conditions. To define the apoptosis-regulatory function of p21, we constructed cells that stably express C-terminal deletion mutants of p21 (full length 164aa), 1-157, 1-147 or 1-128, and evaluated the apoptotic response of these cells. The AnnexinV positive cell fraction after gamma-irradiation did not increase in cells expressing 1-157. Consistently, an increase of caspase3 activity or the active form of caspase3 was not observed in cells expressing 1-157, but was prominent in cells expressing 1-128 and 1-147. Further, the activity of caspase9 was suppressed in gamma-irradiated cells expressing 1-157. The antiapoptotic effect of 1-157 was weaker in Fas-induced apoptosis. Our data indicate that the 1-157 region of p21 inhibits apoptosis caused by gamma-irradiation by reducing the activity of caspase9 and caspase3, and the 148-157 region is critical for its inhibiting activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
431
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-54
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Identification of the region required for the antiapoptotic function of the cyclin kinase inhibitor, p21.
pubmed:affiliation
Department of Biochemistry, Showa University, School of Medicine 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8666, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't