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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
40
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pubmed:dateCreated |
2004-10-7
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pubmed:abstractText |
We demonstrated a microfluidic device for rapidly generating complex mixtures of 32 stock reagents in a 5-nl reactor. This "formulation chip" is fully automated and allows thousands of experiments to be performed in a single day with minimal reagent consumption. It was applied to systematically study the phase behavior of the protein xylanase over a large and complex chemical space. For each chemical formulation that demonstrated a pronounced effect on solubility, the protein phase behavior was completely mapped in the chip, generating a set of empirical phase diagrams. This ab initio phase information was used to devise a rational crystallization screen that resulted in 72-fold improvement in successful crystallization hits compared with conventional sparse matrix screens. This formulations tool allows a physics-based approach to protein crystallization that may prove useful in structural genomics efforts.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-10753110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-12037293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-12351675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-12486223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-12547801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-12705610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-12718926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-12718931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-13517261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-15299385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-15299442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-16220918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-691056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-7827044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-8013449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-8535249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452343-9302288
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
101
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14431-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15452343-Biophysical Phenomena,
pubmed-meshheading:15452343-Biophysics,
pubmed-meshheading:15452343-Chemical Precipitation,
pubmed-meshheading:15452343-Crystallization,
pubmed-meshheading:15452343-Endo-1,4-beta Xylanases,
pubmed-meshheading:15452343-Microfluidics,
pubmed-meshheading:15452343-Proteins,
pubmed-meshheading:15452343-Solubility,
pubmed-meshheading:15452343-Spectrophotometry,
pubmed-meshheading:15452343-Trichoderma
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pubmed:year |
2004
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pubmed:articleTitle |
Systematic investigation of protein phase behavior with a microfluidic formulator.
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pubmed:affiliation |
Department of Applied Physics, California Institute of Technology, MS 128-95, Pasadena, CA 91125, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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