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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
2004-9-28
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pubmed:abstractText |
Expression cloning technology of cDNAs is a suitable tool for identifying novel functional properties of genes. Here, we generated a lentiviral cDNA library-expressing system for human T cells based on a site-specific recombination system of phage lambda for transferring cDNA libraries with a minimum loss of its complexity. The library-transduced CD4(+) T cells were challenged with wild-type human immunodeficiency virus type 1 (HIV-1), and the cells that acquired resistance to HIV-1-induced cytopathic effect (CPE) were selected. From these cells, CD14 was isolated and proved to inhibit the entry of HIV-1 and the HIV-1-induced CPE. This cloning system allows rapid identification of genes encoding novel properties in human T cells and probably other mammalian cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-10077624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-10400815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-10615043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-10873382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-11137994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-11158746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-11219604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-11242119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-11711195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-12034835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-12842440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-12907458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-14501196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-1698311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-2402637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-2437578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-2825196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-3014648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-3501369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-7579669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-8602510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-8876144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-9354796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-9851930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15452256-9924027
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11352-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15452256-Antigens, CD14,
pubmed-meshheading:15452256-Apoptosis,
pubmed-meshheading:15452256-Bacteriophage lambda,
pubmed-meshheading:15452256-CD4-Positive T-Lymphocytes,
pubmed-meshheading:15452256-Cell Line,
pubmed-meshheading:15452256-Cloning, Molecular,
pubmed-meshheading:15452256-Gene Library,
pubmed-meshheading:15452256-Genetic Vectors,
pubmed-meshheading:15452256-HIV-1,
pubmed-meshheading:15452256-Humans,
pubmed-meshheading:15452256-Lentivirus,
pubmed-meshheading:15452256-Recombination, Genetic,
pubmed-meshheading:15452256-Transduction, Genetic
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pubmed:year |
2004
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pubmed:articleTitle |
A lentiviral cDNA library employing lambda recombination used to clone an inhibitor of human immunodeficiency virus type 1-induced cell death.
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pubmed:affiliation |
Department of Virology, Tohoku University Graduate School of Medicine, Sendai, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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