Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1992-4-14
pubmed:abstractText
A quantitative polymerase chain reaction assay was developed that allowed us to monitor transcript levels corresponding to individual members of the cytotoxic cell proteinase (CCP) gene family during T cell activation. Selective expression was observed and shown to depend upon the mode of T cell antigen receptor stimulation. Mitogen or allogeneic stimulation of cells resulted in the appearance of transcripts corresponding to all the genes measured, whereas alpha CD3 antibody produced a response restricted to just two family members. This differential gene activation represents a heterogeneity in cytotoxic T lymphocytes that has not been recognized previously. It may indicate that the T cell branch of the immune system can distinguish between different forms of stimulation and respond by synthesizing a specific set of effector and ancillary molecules that is most appropriate for lysis of cells bearing that type of antigen. Only CCP1 transcripts correlated with cytotoxicity for all modes of stimulation. The patterns for the others are suggestive of distinct and ancillary, rather than direct effector, roles in the lytic mechanism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5090-5
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1544892-Animals, pubmed-meshheading:1544892-Base Sequence, pubmed-meshheading:1544892-Binding Sites, pubmed-meshheading:1544892-Concanavalin A, pubmed-meshheading:1544892-DNA, pubmed-meshheading:1544892-Endopeptidases, pubmed-meshheading:1544892-Gene Expression, pubmed-meshheading:1544892-Gene Expression Regulation, Enzymologic, pubmed-meshheading:1544892-Kinetics, pubmed-meshheading:1544892-Lymphocyte Activation, pubmed-meshheading:1544892-Mice, pubmed-meshheading:1544892-Mice, Inbred BALB C, pubmed-meshheading:1544892-Molecular Sequence Data, pubmed-meshheading:1544892-Oligodeoxyribonucleotides, pubmed-meshheading:1544892-Polymerase Chain Reaction, pubmed-meshheading:1544892-Serine Endopeptidases, pubmed-meshheading:1544892-Spleen, pubmed-meshheading:1544892-T-Lymphocytes, Cytotoxic, pubmed-meshheading:1544892-Transcription, Genetic, pubmed-meshheading:1544892-Transcriptional Activation
pubmed:year
1992
pubmed:articleTitle
Quantitative polymerase chain reaction analysis of cytotoxic cell proteinase gene transcripts in T cells. Pattern of expression is dependent on the nature of the stimulus.
pubmed:affiliation
Department of Biochemistry, University of Alberta, Edmonton, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't