Linked Life Data

15380106

Source:http://linkedlifedata.com/resource/pubmed/id/15380106

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2004-9-21
pubmed:abstractText
The progress of replicative DNA polymerases along the replication fork may be impeded by the presence of lesions in the genome. One way to circumvent such hurdles involves the recruitment of specialized DNA polymerases that perform limited incorporation of nucleotides in the vicinity of the damaged site. This process entails DNA polymerase switch between replicative and specialized DNA polymerases. Five eukaryotic proteins can carry out translesion synthesis (TLS) of damaged DNA in vitro, DNA polymerases zeta, eta, iota, and kappa, and REV1. To identify novel proteins that interact with hpol eta, we performed a yeast two-hybrid screen. In this paper, we show that hREV1 interacts with hpol eta as well as with hpol kappa and poorly with hpol iota. Furthermore, cellular localization analysis demonstrates that hREV1 is present, with hpol eta in replication factories at stalled replication forks and is tightly associated with nuclear structures. This hREV1 nuclear localization occurs independently of the presence of hpol eta. Taken together, our data suggest a central role for hREV1 as a scaffold that recruits DNA polymerases involved in TLS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1568-7864
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1503-14
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Co-localization in replication foci and interaction of human Y-family members, DNA polymerase pol eta and REVl protein.
pubmed:affiliation
UPR9OO3 - CNRS, Cancérogenèse et Mutagenèse Moléculaire et Structurale, ESBS, Pole API/Bd Sébastien Brant BP 10413, F-67412 - ILLKIRCH, France. tissier@esbs.u-strasbg.fr
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't