Source:http://linkedlifedata.com/resource/pubmed/id/15373569
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2004-9-17
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pubmed:abstractText |
Pretransfusion tests must detect antibodies that can shorten the life of red blood cells (RBCs). Some studies have demonstrated the existence of clinically significant antibodies detected at 37 degrees C in saline that are not detected by the indirect antiglobulin test (IAT) when the conventional tube test is used. Our aim was to determine whether these antibodies, detected with a 37 degrees C saline tube test, are also detected when a sensitive column gel agglutination method is used. The 2373 pretransfusion samples were tested as they were received (from May 1998 to December 1999), in 37 degrees C saline and by IAT using the DiaMed gel system. The screening tests were performed using 50 microL of 0.8% low-ionic-strength saline suspended RBCs and 50 microL of plasma. The tests were examined for agglutination and hemolysis. Two hundred and thirty three samples (9.81%) were reactive by IAT and 88 (3.70%) by 37 degrees C saline. All 88 samples reactive by 37 degrees C saline also reacted by IAT. These data indicate that 37 degrees C saline is not an essential pretransfusion procedure when the DiaMed gel test is used.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:status |
PubMed-not-MEDLINE
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pubmed:issn |
0894-203X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13-5
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pubmed:dateRevised |
2008-4-8
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pubmed:year |
2002
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pubmed:articleTitle |
Antibody screening in 37 degrees C saline. Is it safe to omit it using the indirect antiglobulin (gel) test?
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pubmed:affiliation |
Laboratory of Immunohematology, Centro Regional de Sangue do Porto, Estrada da Circunvalação, Portugal.
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pubmed:publicationType |
Journal Article
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