Source:http://linkedlifedata.com/resource/pubmed/id/15372255
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
2004-9-16
|
| pubmed:abstractText |
Activated myelin-specific T cells are thought to mediate inflammatory tissue damage in multiple sclerosis (MS). Applying a large panel of myelin antigens, we demonstrate the direct ex vivo detection of viable IFN-gamma/TNF-alpha producing CD4+/CD69+ T cells 6 hours after antigenic challenge, by intracellular flow cytometry in 3/33 MS patients and 2/26 healthy controls with calculated frequencies of (mean +/- SEM): 0.031% +/- 0.002% versus 0.037% +/- 0.029%. By comparison, the recently developed IL-7 modified proliferation assay revealed i) a higher number of individuals showing myelin reactivity (17/37 MS patients and 12/24 healthy individuals) and ii) a significant difference in the response to myelin basic protein (MBP) between the two groups in a longitudinal analysis, indicating a higher activity of myelin-specific T cells in MS patients. Our data provide new perspectives in detecting pathogenetically relevant T cells, but clearly demonstrate the different conclusions which must be drawn from various approaches concerning the quantification of autoreactive T cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0340-5354
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
251
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1111-20
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15372255-Adult,
pubmed-meshheading:15372255-Amino Acid Sequence,
pubmed-meshheading:15372255-Autoantigens,
pubmed-meshheading:15372255-Binding Sites,
pubmed-meshheading:15372255-Cell Separation,
pubmed-meshheading:15372255-Cross-Sectional Studies,
pubmed-meshheading:15372255-Female,
pubmed-meshheading:15372255-Flow Cytometry,
pubmed-meshheading:15372255-Humans,
pubmed-meshheading:15372255-Longitudinal Studies,
pubmed-meshheading:15372255-Lymphocyte Activation,
pubmed-meshheading:15372255-Male,
pubmed-meshheading:15372255-Middle Aged,
pubmed-meshheading:15372255-Molecular Sequence Data,
pubmed-meshheading:15372255-Multiple Sclerosis,
pubmed-meshheading:15372255-Myelin Basic Proteins,
pubmed-meshheading:15372255-Statistics, Nonparametric,
pubmed-meshheading:15372255-T-Lymphocytes
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Cross-sectional and longitudinal analysis of myelin-reactive T cells in patients with multiple sclerosis.
|
| pubmed:affiliation |
Institute of Neuroimmunology, Neuroscience Research Center, Charité, 10098, Berlin, Germany.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|