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pubmed-article:15367649pubmed:abstractTextThe regulation of TT virus (TTV) gene expression was characterized. Transient-transfection assays using reporter constructs revealed that a 113-nucleotide (nt) sequence within the untranslated region, proximal to the transcription initiation site and containing a TATA box motif, has a basal promoter activity. This sequence is well conserved among different TTV genotypes. Upstream stimulating factor bound to a consensus binding motif within this region and positively regulates TTV transcription. Furthermore, a 488-nt region upstream of the basal promoter exhibited enhancer activity, presumably in a cell type-specific manner. This study illustrates some of the mechanisms involved in the transcriptional regulation of TTV.lld:pubmed
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pubmed-article:15367649pubmed:articleTitleIdentification of basal promoter and enhancer elements in an untranslated region of the TT virus genome.lld:pubmed
pubmed-article:15367649pubmed:affiliationDepartment of Virology II, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. tesuzuki@nih.go.jplld:pubmed
pubmed-article:15367649pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:15367649pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed