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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
2004-9-15
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pubmed:abstractText |
Infection of receptor-bearing cells by coronaviruses is mediated by their spike (S) proteins. The coronavirus (SARS-CoV) that causes severe acute respiratory syndrome (SARS) infects cells expressing the receptor angiotensin-converting enzyme 2 (ACE2). Here we show that codon optimization of the SARS-CoV S-protein gene substantially enhanced S-protein expression. We also found that two retroviruses, simian immunodeficiency virus (SIV) and murine leukemia virus, both expressing green fluorescent protein and pseudotyped with SARS-CoV S protein or S-protein variants, efficiently infected HEK293T cells stably expressing ACE2. Infection mediated by an S-protein variant whose cytoplasmic domain had been truncated and altered to include a fragment of the cytoplasmic tail of the human immunodeficiency virus type 1 envelope glycoprotein was, in both cases, substantially more efficient than that mediated by wild-type S protein. Using S-protein-pseudotyped SIV, we found that the enzymatic activity of ACE2 made no contribution to S-protein-mediated infection. Finally, we show that a soluble and catalytically inactive form of ACE2 potently blocked infection by S-protein-pseudotyped retrovirus and by SARS-CoV. These results permit studies of SARS-CoV entry inhibitors without the use of live virus and suggest a candidate therapy for SARS.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-10089882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-10497246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-10725213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-11069993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-12358520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-12606557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-12690091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-12690092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-12730500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-12730501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-12748632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-12823946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-12887918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-12892955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-12927536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-14647384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-14651994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-14766227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-15016880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-15024391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-2204064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-7745730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-7913510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-8674119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15367630-8805248
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-538X
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pubmed:author |
pubmed-author:ChoeHyeryunH,
pubmed-author:CoderreJamesJ,
pubmed-author:DorfmanTatyanaT,
pubmed-author:FarzanMichaelM,
pubmed-author:GreenoughThomas CTC,
pubmed-author:HanZhongchaoZ,
pubmed-author:KuhnJens HJH,
pubmed-author:LiWenhuiW,
pubmed-author:LiYanhanY,
pubmed-author:MooreMichael JMJ,
pubmed-author:VasilievaNatalyaN,
pubmed-author:WongSwee KeeSK
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10628-35
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15367630-Amino Acid Sequence,
pubmed-meshheading:15367630-Animals,
pubmed-meshheading:15367630-Carboxypeptidases,
pubmed-meshheading:15367630-Cell Line,
pubmed-meshheading:15367630-HIV-1,
pubmed-meshheading:15367630-Humans,
pubmed-meshheading:15367630-Leukemia Virus, Murine,
pubmed-meshheading:15367630-Membrane Glycoproteins,
pubmed-meshheading:15367630-Molecular Sequence Data,
pubmed-meshheading:15367630-Peptidyl-Dipeptidase A,
pubmed-meshheading:15367630-Receptors, Virus,
pubmed-meshheading:15367630-SARS Virus,
pubmed-meshheading:15367630-Simian immunodeficiency virus,
pubmed-meshheading:15367630-Viral Envelope Proteins,
pubmed-meshheading:15367630-Virion,
pubmed-meshheading:15367630-Virus Replication
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pubmed:year |
2004
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pubmed:articleTitle |
Retroviruses pseudotyped with the severe acute respiratory syndrome coronavirus spike protein efficiently infect cells expressing angiotensin-converting enzyme 2.
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pubmed:affiliation |
Partners AIDS Research Center, Brigham and Women's Hospital, and Department of Medicine, Harvard Medical School, Boston, MA, USA.
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pubmed:publicationType |
Journal Article
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