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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2004-9-14
pubmed:databankReference
pubmed:abstractText
Three ASF/SF2-like alternative splicing genes from maize were identified, cloned, and analyzed. Each of these genes (zmSRp30, zmSRp31, and zmSRp32) contains two RNA binding domains, a signature sequence SWQDLKD, and a characteristic serine/ariginine-rich domain. There is a strong structural similarity to the human ASF/SF2 splicing factor and to the Arabidopsis atSRp34/p30 proteins. Similar to ASF/SF2-like genes in other organisms, the maize pre-mRNA messages are alternatively spliced. They are differentially expressed in maize tissues with relatively uniform levels of zmSRp30 and zmSRp31 messages being observed throughout the plant, while zmSRp32 messages preferentially accumulated in the meristematic regions. Overexpression of zmSRp32 in maize cells leads to the enhanced selection of weak 5' intron splice sites during the processing of pre-mRNA molecules. Overexpression of the zmSRp31 or zmSRp32 gene affects regulation of wheat dwarf virus rep gene pre-mRNA splicing, presumably by interacting with the weak 5' splice site, CCGU. Our results suggest that the described genes are functional homologues of the human ASF/SF2 alternative splicing factor and they indicate a diversity of the ASF/SF2-like alternative splicing factors in monocot plant cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
339
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
25-37
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15363843-Alternative Splicing, pubmed-meshheading:15363843-Amino Acid Sequence, pubmed-meshheading:15363843-Base Sequence, pubmed-meshheading:15363843-Cloning, Molecular, pubmed-meshheading:15363843-DNA, Plant, pubmed-meshheading:15363843-Gene Expression Regulation, Developmental, pubmed-meshheading:15363843-Gene Expression Regulation, Plant, pubmed-meshheading:15363843-Molecular Sequence Data, pubmed-meshheading:15363843-Multigene Family, pubmed-meshheading:15363843-Phylogeny, pubmed-meshheading:15363843-Plant Proteins, pubmed-meshheading:15363843-Protein Isoforms, pubmed-meshheading:15363843-RNA Precursors, pubmed-meshheading:15363843-RNA Splicing, pubmed-meshheading:15363843-RNA-Binding Proteins, pubmed-meshheading:15363843-Seeds, pubmed-meshheading:15363843-Sequence Alignment, pubmed-meshheading:15363843-Sequence Analysis, DNA, pubmed-meshheading:15363843-Sequence Homology, Amino Acid, pubmed-meshheading:15363843-Transcription, Genetic, pubmed-meshheading:15363843-Zea mays
pubmed:year
2004
pubmed:articleTitle
ASF/SF2-like maize pre-mRNA splicing factors affect splice site utilization and their transcripts are alternatively spliced.
pubmed:affiliation
Pioneer Hi-Bred International (a DuPont company), Johnston, IA, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't