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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1992-8-6
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pubmed:abstractText |
A cellular phosphoprotein with an apparent molecular mass of 90 kd (p90) that forms a complex with both mutant and wild-type p53 protein has been characterized, purified, and identified. The protein was identified as a product of the murine double minute 2 gene (mdm-2). The mdm-2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. To determine if mdm-2 could modulate p53 transactivation, a p53-responsive element from the muscle creatine kinase gene was employed. A wild-type p53-expressing plasmid enhanced the expression of the p53-responsive element when cotransfected into cells that contain no endogenous p53. When a cosmid expressing mdm-2 was transfected with this p53-expressing plasmid, the transactivation of the p53-responsive element was inhibited. Thus, a product of the mdm-2 oncogene forms a tight complex with the p53 protein, and the mdm-2 oncogene can inhibit p53-mediated transactivation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
69
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pubmed:geneSymbol |
mdm-2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1237-45
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1535557-Amino Acid Sequence,
pubmed-meshheading:1535557-Animals,
pubmed-meshheading:1535557-Base Sequence,
pubmed-meshheading:1535557-Cell Line,
pubmed-meshheading:1535557-Mice,
pubmed-meshheading:1535557-Molecular Sequence Data,
pubmed-meshheading:1535557-Molecular Weight,
pubmed-meshheading:1535557-Neoplasm Proteins,
pubmed-meshheading:1535557-Nuclear Proteins,
pubmed-meshheading:1535557-Oncogene Proteins,
pubmed-meshheading:1535557-Plasmids,
pubmed-meshheading:1535557-Protein Binding,
pubmed-meshheading:1535557-Proto-Oncogene Proteins,
pubmed-meshheading:1535557-Proto-Oncogene Proteins c-mdm2,
pubmed-meshheading:1535557-Rats,
pubmed-meshheading:1535557-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1535557-Transcriptional Activation,
pubmed-meshheading:1535557-Tumor Suppressor Protein p53
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pubmed:year |
1992
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pubmed:articleTitle |
The mdm-2 oncogene product forms a complex with the p53 protein and inhibits p53-mediated transactivation.
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pubmed:affiliation |
Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, New Jersey 08544-1014.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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