|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2004-8-18
|
|
pubmed:abstractText |
The transcription factor NF-kappaB regulates a wide variety of genes involved in multiple processes. Although the apparent consensus sequence of DNA binding sites for NF-kappaB (kappaB sites) is very broad, the sites active in any one gene show remarkable evolutionary stability. Using a lentivirus-based methodology for implantation of gene regulatory sequences we show that for genes with two kappaB sites, both are required for activity. Swapping sites between kappaB-dependent genes altered NF-kappaB dimer specificity of the promoters and revealed that two kappaB sites can function together as a module to regulate gene activation. Further, although the sequence of the kappaB site is important for determining kappaB family member specificity, rather than determining the ability of a particular dimer to bind effectively, the sequence affects which coactivators will form productive interactions with the bound NF-kappaB dimer. This suggests that binding sites may impart a specific configuration to bound transcription factors.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL10,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/proto-oncogene protein bcl-3
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0092-8674
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
20
|
|
pubmed:volume |
118
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
453-64
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:15315758-Animals,
pubmed-meshheading:15315758-Base Sequence,
pubmed-meshheading:15315758-Binding Sites,
pubmed-meshheading:15315758-Chemokine CXCL10,
pubmed-meshheading:15315758-Chemokines, CXC,
pubmed-meshheading:15315758-Chromatin,
pubmed-meshheading:15315758-Conserved Sequence,
pubmed-meshheading:15315758-DNA,
pubmed-meshheading:15315758-Dimerization,
pubmed-meshheading:15315758-Fibroblasts,
pubmed-meshheading:15315758-Genes, Reporter,
pubmed-meshheading:15315758-Humans,
pubmed-meshheading:15315758-Lentivirus,
pubmed-meshheading:15315758-Mice,
pubmed-meshheading:15315758-Models, Biological,
pubmed-meshheading:15315758-Molecular Sequence Data,
pubmed-meshheading:15315758-NF-kappa B,
pubmed-meshheading:15315758-Plasmids,
pubmed-meshheading:15315758-Precipitin Tests,
pubmed-meshheading:15315758-Promoter Regions, Genetic,
pubmed-meshheading:15315758-Protein Binding,
pubmed-meshheading:15315758-Proto-Oncogene Proteins,
pubmed-meshheading:15315758-RNA, Small Interfering,
pubmed-meshheading:15315758-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15315758-Time Factors,
pubmed-meshheading:15315758-Transcription Factors,
pubmed-meshheading:15315758-Transgenes
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
One nucleotide in a kappaB site can determine cofactor specificity for NF-kappaB dimers.
|
|
pubmed:affiliation |
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
