Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-3-24
pubmed:abstractText
Neutrophil-activating peptide 2 (NAP-2), corresponding to platelet basic protein fragment 25-94, was prepared by chymotryptic digestion of its precursors, low affinity platelet factor 4 or beta-thromboglobulin, followed by purification by high performance liquid chromatography. NAP-2 (0.1-1.5 microns) caused the release of human granulocyte elastase from cytochalasin B-treated neutrophils in a dose-dependent manner. In the same system, beta-thromboglobulin, human platelet factor 4, S-pyridylethyl NAP-2, and platelet basic protein C-terminal fragment (77-94) were inactive, whereas platelet basic protein fragment 22-89 had low, but significant, activity. Sensitive immunological identification of NAP-2 based on nonequilibrium isoelectric focusing and immunoblotting is described.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Blood Coagulation Factors, http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, http://linkedlifedata.com/resource/pubmed/chemical/Cytochalasin B, http://linkedlifedata.com/resource/pubmed/chemical/Heparin Antagonists, http://linkedlifedata.com/resource/pubmed/chemical/Leukocyte Elastase, http://linkedlifedata.com/resource/pubmed/chemical/PPBP protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Pancreatic Elastase, http://linkedlifedata.com/resource/pubmed/chemical/Peptides, http://linkedlifedata.com/resource/pubmed/chemical/Platelet Factor 4, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/beta-Thromboglobulin, http://linkedlifedata.com/resource/pubmed/chemical/connective tissue-activating peptide, http://linkedlifedata.com/resource/pubmed/chemical/low affinity platelet factor 4
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0037-9727
pubmed:author
pubmed:issnType
Print
pubmed:volume
199
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
171-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1531540-Amino Acid Sequence, pubmed-meshheading:1531540-Blood Coagulation Factors, pubmed-meshheading:1531540-Chemokines, pubmed-meshheading:1531540-Chromatography, High Pressure Liquid, pubmed-meshheading:1531540-Cytochalasin B, pubmed-meshheading:1531540-Dose-Response Relationship, Drug, pubmed-meshheading:1531540-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:1531540-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:1531540-Heparin Antagonists, pubmed-meshheading:1531540-Humans, pubmed-meshheading:1531540-Immunoblotting, pubmed-meshheading:1531540-Isoelectric Focusing, pubmed-meshheading:1531540-Leukocyte Elastase, pubmed-meshheading:1531540-Molecular Sequence Data, pubmed-meshheading:1531540-Neutrophils, pubmed-meshheading:1531540-Pancreatic Elastase, pubmed-meshheading:1531540-Peptides, pubmed-meshheading:1531540-Platelet Factor 4, pubmed-meshheading:1531540-Proteins, pubmed-meshheading:1531540-Sequence Homology, Nucleic Acid, pubmed-meshheading:1531540-beta-Thromboglobulin
pubmed:year
1992
pubmed:articleTitle
Isolation, characterization, and immunological detection of neutrophil-activating peptide 2: a proteolytic degradation product of platelet basic protein.
pubmed:affiliation
Rhône-Poulenc Rorer Biotechnology, Inc., King of Prussia, Pennsylvania 19406.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.