Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
34
pubmed:dateCreated
2004-8-25
pubmed:abstractText
Our understanding of mechanisms by which the expression of IFN-gamma is regulated is limited. Herein, we identify two evolutionarily conserved noncoding sequence elements (IFNgCNS1 and IFNg CNS2) located approximately 5 kb upstream and approximately 18 kb downstream of the initiation codon of the murine Ifng gene. When linked to the murine Ifng gene (-3.4 to +5.6 kb) and transiently transfected into EL-4 cells, these elements clearly enhanced IFN-gamma expression in response to ionomycin and phorbol 12-myristate 13-acetate and weakly enhanced expression in response to T-bet. A DNase I hypersensitive site and extragenic transcripts at IFNgCNS2 correlated positively with the capacity of primary T cell subsets to produce IFN-gamma. Transcriptionally favorable histone modifications in the Ifng promoter, intronic regions, IFNgCNS2, and, although less pronounced, IFNgCNS1 increased as naïve T cells differentiated into IFN-gamma-producing effector CD8+ and T helper (TH) 1 T cells, but not into TH2 T cells. Like IFN-gamma expression, these histone modifications were T-bet-dependent in CD4+ cells, but not CD8+ T cells. These findings define two distal regulatory elements associated with T cell subset-specific IFN-gamma expression.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-10753117, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-10761931, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-10882078, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-10984448, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-11397944, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-11441092, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-11526400, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-11786644, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-11997340, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-12055628, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-12097365, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-12384402, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-12471094, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-12524537, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-12697725, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-12861010, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-12932361, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-13679385, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-14595437, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-14605368, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-14607827, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-14691261, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-1904081, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-6327064, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-8228802, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-8972200, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-9334315, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-9846495, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-9857002, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-9881967, http://linkedlifedata.com/resource/pubmed/commentcorrection/15304658-9885919
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12622-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:15304658-Animals, pubmed-meshheading:15304658-Cells, Cultured, pubmed-meshheading:15304658-Evolution, Molecular, pubmed-meshheading:15304658-Gene Expression Regulation, pubmed-meshheading:15304658-Histones, pubmed-meshheading:15304658-Humans, pubmed-meshheading:15304658-Interferon-gamma, pubmed-meshheading:15304658-Mice, pubmed-meshheading:15304658-Mice, Inbred BALB C, pubmed-meshheading:15304658-Mice, Inbred C57BL, pubmed-meshheading:15304658-Mice, Knockout, pubmed-meshheading:15304658-Protein Processing, Post-Translational, pubmed-meshheading:15304658-Rats, pubmed-meshheading:15304658-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:15304658-T-Box Domain Proteins, pubmed-meshheading:15304658-T-Lymphocytes, pubmed-meshheading:15304658-Transcription, Genetic, pubmed-meshheading:15304658-Transcription Factors
pubmed:year
2004
pubmed:articleTitle
Evolutionarily conserved sequence elements that positively regulate IFN-gamma expression in T cells.
pubmed:affiliation
Department of Immunology, University of Washington, Seattle, WA 98195, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.