Linked Life Data

15292233

Source:http://linkedlifedata.com/resource/pubmed/id/15292233

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
42
pubmed:dateCreated
2004-10-11
pubmed:databankReference
pubmed:abstractText
The Xvent-2B promoter is regulated by a BMP-2/4-induced transcription complex comprising Smad signal transducers and specific transcription factors. Using a yeast one-hybrid screen we have found that Oct-25, a Xenopus POU domain protein related to mammalian Oct-3/4, binds as an additional factor to the Xvent-2B promoter. This interaction was further confirmed by both in vitro and in vivo analyses. The Oct-25 gene is mainly transcribed during blastula and gastrula stages in the newly forming ectodermal and mesodermal germ layers. Luciferase reporter gene assay demonstrated that Oct-25 stimulates transcription of the Xvent-2B gene. This stimulation depends on the Oct-25 binding site and the bone morphogenetic protein-responsive element. Furthermore, Oct-25 interacts in vitro with components of the Xvent-2B transcription complex, like Smad1/4 and Xvent-2. Overexpression of Oct-25 results in anterior/posterior truncations and lack of differentiation for neuroectoderm- and mesoderm-derived tissues including blood cells. This effect is consistent with an evolutionarily conserved role of class V POU factors in the maintenance of an undifferentiated cell state. In Xenopus, the molecular mechanism underlying this process might be coupled to the expression of Xvent proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
279
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
43735-43
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15292233-Animals, pubmed-meshheading:15292233-Base Sequence, pubmed-meshheading:15292233-Blastomeres, pubmed-meshheading:15292233-Cell Differentiation, pubmed-meshheading:15292233-Cloning, Molecular, pubmed-meshheading:15292233-DNA Footprinting, pubmed-meshheading:15292233-DNA Primers, pubmed-meshheading:15292233-Deoxyribonuclease I, pubmed-meshheading:15292233-Embryo, Nonmammalian, pubmed-meshheading:15292233-Gene Expression Regulation, Developmental, pubmed-meshheading:15292233-Genes, Reporter, pubmed-meshheading:15292233-Homeodomain Proteins, pubmed-meshheading:15292233-Molecular Sequence Data, pubmed-meshheading:15292233-Promoter Regions, Genetic, pubmed-meshheading:15292233-Transcription Factors, pubmed-meshheading:15292233-Xenopus Proteins, pubmed-meshheading:15292233-Xenopus laevis
pubmed:year
2004
pubmed:articleTitle
The POU factor Oct-25 regulates the Xvent-2B gene and counteracts terminal differentiation in Xenopus embryos.
pubmed:affiliation
Abteilung Biochemie, Universität Ulm, Albert-Einstein-Allee 11, D-89081, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't