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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2004-7-26
pubmed:databankReference
pubmed:abstractText
GCN2 is the alpha-subunit of the only translation initiation factor (eIF2alpha) kinase that appears in all eukaryotes. Its function requires an interaction with GCN1 via the domain at its N-terminus, which is termed the RWD domain after three major RWD-containing proteins: RING finger-containing proteins, WD-repeat-containing proteins, and yeast DEAD (DEXD)-like helicases. In this study, we determined the solution structure of the mouse GCN2 RWD domain using NMR spectroscopy. The structure forms an alpha + beta sandwich fold consisting of two layers: a four-stranded antiparallel beta-sheet, and three side-by-side alpha-helices, with an alphabetabetabetabetaalphaalpha topology. A characteristic YPXXXP motif, which always occurs in RWD domains, forms a stable loop including three consecutive beta-turns that overlap with each other by two residues (triple beta-turn). As putative binding sites with GCN1, a structure-based alignment allowed the identification of several surface residues in alpha-helix 3 that are characteristic of the GCN2 RWD domains. Despite the apparent absence of sequence similarity, the RWD structure significantly resembles that of ubiquitin-conjugating enzymes (E2s), with most of the structural differences in the region connecting beta-strand 4 and alpha-helix 3. The structural architecture, including the triple beta-turn, is fundamentally common among various RWD domains and E2s, but most of the surface residues on the structure vary. Thus, it appears that the RWD domain is a novel structural domain for protein-binding that plays specific roles in individual RWD-containing proteins.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-10066441, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-10212987, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-10500182, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-10500305, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-10504407, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-10655230, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-10775272, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-10801780, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-10869041, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-10983975, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-11101534, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-11217851, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-11250908, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-11265246, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-11276253, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-11350982, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-11440714, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-11779830, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-11786179, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-12215525, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-12455968, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-12484774, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-12499312, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-12522306, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-1960729, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-2038314, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-7621831, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-7623840, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-7756980, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-7966293, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-8234246, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-8377180, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-8520220, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-8744573, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-8798780, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-9008363, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-9067611, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-9139706, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-9254694, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-9261152, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-9367762, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-9430731, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-9514257, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-9649537, http://linkedlifedata.com/resource/pubmed/commentcorrection/15273307-9923595
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0961-8368
pubmed:author
pubmed-author:AokiMasaakiM, pubmed-author:ArakawaTakahiroT, pubmed-author:CarninciPieroP, pubmed-author:GüntertPeterP, pubmed-author:HaradaTakushiT, pubmed-author:HayashizakiYoshihideY, pubmed-author:HirotaHiroshiH, pubmed-author:InoueMakotoM, pubmed-author:KawaiJunJ, pubmed-author:KigawaTakanoriT, pubmed-author:KinoshitaKengoK, pubmed-author:KobayashiNaohiroN, pubmed-author:KoshibaSeizoS, pubmed-author:MatsudaNatsukoN, pubmed-author:MatsudaTakayoshiT, pubmed-author:NamekiNobukazuN, pubmed-author:NunokawaEmiE, pubmed-author:OsanaiTakashiT, pubmed-author:SaitoKoheiK, pubmed-author:SakagamiNorikoN, pubmed-author:SekiEikoE, pubmed-author:ShirouzuMikakoM, pubmed-author:TanakaAkikoA, pubmed-author:TeradaTakahoT, pubmed-author:TochioNaoyaN, pubmed-author:TomoYasukoY, pubmed-author:YabukiTakashiT, pubmed-author:YokoyamaShigeyukiS, pubmed-author:YoneyamaMisaoM, pubmed-author:YoshidaMayumiM
pubmed:issnType
Print
pubmed:volume
13
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