rdf:type |
|
lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-10-20
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pubmed:abstractText |
A glucose-specific lectin has been purified to apparent homogeneity from 7-day-old peanut (Arachis hypogaea) roots by affinity chromatography on a Sephadex G-50. The lectin has a 66 kDa native molecular mass and a 33 kDa subunit molecular mass as revealed by native and denaturing sodium dodecyl sulphate-polyacrylamide gel electrophoresis, respectively. The purified lectin, gives a single precipitin line with the antiserum produced against 7-day-old root extract and shows 5 bands in the pH range of 4.4-5.4 in the isoelectric focusing gel. The glucose-specific lectin activity in the peanut roots appears from the fourth day onwards. Lipopolysaccharides isolated from the host specific Rhizobium strain are a 68-fold more potent inhibitor of the lectin as compared to glucose.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Sep
|
pubmed:issn |
0006-3002
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
1117
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
114-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1525173-Animals,
pubmed-meshheading:1525173-Arachis hypogaea,
pubmed-meshheading:1525173-Chromatography, Affinity,
pubmed-meshheading:1525173-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1525173-Glucose,
pubmed-meshheading:1525173-Hemagglutination,
pubmed-meshheading:1525173-Humans,
pubmed-meshheading:1525173-Hydrogen-Ion Concentration,
pubmed-meshheading:1525173-Isoelectric Focusing,
pubmed-meshheading:1525173-Lectins,
pubmed-meshheading:1525173-Manganese,
pubmed-meshheading:1525173-Molecular Weight,
pubmed-meshheading:1525173-Neuraminidase,
pubmed-meshheading:1525173-Peanut Agglutinin,
pubmed-meshheading:1525173-Plant Lectins,
pubmed-meshheading:1525173-Rabbits
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pubmed:year |
1992
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pubmed:articleTitle |
Isolation and characterization of a lectin from peanut roots.
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pubmed:affiliation |
C.S.I.R. Centre for Biochemicals, Delhi University Campus, India.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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