Linked Life Data

15242938

Source:http://linkedlifedata.com/resource/pubmed/id/15242938

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-7-9
pubmed:abstractText
By using a recombinant severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein-based enzyme-linked immunosorbent assay (ELISA) and serum specimens serially collected (from day 0 to day 240 after symptom onset) from patients with pneumonia due to SARS-CoV, we analyzed the longitudinal profiles of immunoglobulin G (IgG), IgM, and IgA antibodies against the SARS-CoV nucleocapsid protein in patients with pneumonia due to SARS-CoV. For IgG, the median optical density at 450 nm (OD450) turned positive at day 17 and a biphasic response was observed. At day 240, all patients were still positive for anti-nucleocapsid protein IgG antibody. For IgM, the median OD450 turned positive at day 20.5, peaked at about day 80, and fell to below the baseline level at about day 180. At day 240, 36% of the patients were still positive for anti-nucleocapsid protein IgM antibody. For IgA, the median OD450 turned positive at day 17, peaked at about day 50, and fell to below the baseline level at about day 180. At day 240, 36% of the patients were still positive for anti-nucleocapsid protein IgA antibody. The time of seroconversion detected by the recombinant SARS-CoV nucleocapsid protein-based ELISA and that detected by indirect immunofluorescence assay were similar. The median times of seroconversion for IgG, IgM, and IgA detected by the indirect immunofluorescence assay were 17 days (17 days by ELISA), 16.5 days (20.5 days by ELISA), and 17.5 days (17 days by ELISA), respectively, after disease onset. One, four, and one of the six patients who died did not produce any IgG, IgM, and IgA antibodies against the nucleocapsid protein of SARS-CoV, respectively, although these antibodies were detected in all six patients by the indirect immunofluorescence assay. Further studies should be performed to see whether SARS-CoV nucleocapsid protein antibody positivity has any prognostic significance.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15242938-11682494, http://linkedlifedata.com/resource/pubmed/commentcorrection/15242938-12037061, http://linkedlifedata.com/resource/pubmed/commentcorrection/15242938-12574298, http://linkedlifedata.com/resource/pubmed/commentcorrection/15242938-12690092, http://linkedlifedata.com/resource/pubmed/commentcorrection/15242938-12711465, http://linkedlifedata.com/resource/pubmed/commentcorrection/15242938-12781535, http://linkedlifedata.com/resource/pubmed/commentcorrection/15242938-12890855, http://linkedlifedata.com/resource/pubmed/commentcorrection/15242938-12958366, http://linkedlifedata.com/resource/pubmed/commentcorrection/15242938-14681510, http://linkedlifedata.com/resource/pubmed/commentcorrection/15242938-15031027, http://linkedlifedata.com/resource/pubmed/commentcorrection/15242938-15131220, http://linkedlifedata.com/resource/pubmed/commentcorrection/15242938-7714191
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1071-412X
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
665-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Longitudinal profile of immunoglobulin G (IgG), IgM, and IgA antibodies against the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in patients with pneumonia due to the SARS coronavirus.
pubmed:affiliation
Department of Microbiology, The University of Hong Kong, Hong Kong.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't