Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-7-5
pubmed:abstractText
Neuropathic pain after spinal cord injury (SCI) represents a difficult problem that is commonly refractory to conventional medical management. To determine if spinal release of gamma-amino butyric acid (GABA) could reduce below-level central neuropathic pain after SCI, we constructed a replication-incompetent herpes simplex virus (HSV)-based vector encoding one isoform of human glutamic acid decarboxylase (GAD67). Dorsal root ganglion (DRG) neurons transduced in vitro or in vivo by subcutaneous inoculation produced GAD and released GABA constitutively. T13 spinal cord hemisection resulted in central neuropathic pain manifested by mechanical allodynia and thermal hyperalgesia. Subcutaneous inoculation of the vector into both feet reduced both manifestations of below-level SCI pain; the vector-mediated effect was partially reversed by intrathecal bicuculline or phaclofen at doses that did not affect thresholds in normal or injured uninoculated animals. Vector-mediated GABA release attenuated the increase in spinal calcitonin gene-related peptide immunoreactivity caused by cord hemisection. These results suggest that HSV-mediated gene transfer to DRG could be used to treat below-level central neuropathic pain after incomplete SCI.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1525-0016
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
57-66
pubmed:dateRevised
2007-10-24
pubmed:meshHeading
pubmed-meshheading:15233942-Animals, pubmed-meshheading:15233942-Baclofen, pubmed-meshheading:15233942-Bicuculline, pubmed-meshheading:15233942-Calcitonin Gene-Related Peptide, pubmed-meshheading:15233942-Ganglia, Spinal, pubmed-meshheading:15233942-Gene Therapy, pubmed-meshheading:15233942-Genetic Vectors, pubmed-meshheading:15233942-Glutamate Decarboxylase, pubmed-meshheading:15233942-Hyperalgesia, pubmed-meshheading:15233942-Injections, Subcutaneous, pubmed-meshheading:15233942-Isoenzymes, pubmed-meshheading:15233942-Neurons, pubmed-meshheading:15233942-Pain, pubmed-meshheading:15233942-Rats, pubmed-meshheading:15233942-Simplexvirus, pubmed-meshheading:15233942-Spinal Cord Injuries, pubmed-meshheading:15233942-Tissue Distribution, pubmed-meshheading:15233942-Transduction, Genetic, pubmed-meshheading:15233942-gamma-Aminobutyric Acid
pubmed:year
2004
pubmed:articleTitle
Peripherally delivered glutamic acid decarboxylase gene therapy for spinal cord injury pain.
pubmed:affiliation
Department of Neurology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't