Linked Life Data

15202936

Source:http://linkedlifedata.com/resource/pubmed/id/15202936

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
2005-3-18
pubmed:abstractText
To improve the antisense activity of AS ODN (antisense oligodeoxynucleotide), a conjugate covalently linked to DOX (doxorubicin) at its 3'-end was synthesized and its antisense activity in human carcinoma DOX-resistant cells (KB-A-1) was investigated in vitro. The intracellular DOX concentration in KB-A-1 cells treated with the conjugate was detected in vitro by HPLC. Results showed that the intracellular DOX concentration was 6.4-fold higher in KB-A-1 cells treated with the conjugate when compared with that in the cells treated with DOX alone. In contrast, a 1.8-fold increase in the concentration of DOX was observed when the cells were treated with AS ODN. Reverse transcriptase PCR and Western-blot analysis showed a more significant decrease in the amount of mdr1 (multidrug resistance 1 gene) mRNA and P-glycoprotein in KB-A-1 cells. Chemosensitivity of KB-A-1 cells to DOX was also investigated in vitro. When the cells were first exposed to the conjugate (0.5 microM) for 24 h and then exposed to DOX for 24 h, the IC(50) value of DOX decreased from 21.5 to 2.2 microM, whereas the IC(50) value of DOX decreased only to 16.8 microM when the cells were treated with the mixture of the same concentration of AS ODN. These results suggest that the conjugate is effective in reversing multidrug resistance. Further studies will be conducted to explore the effect of the conjugate on tumours in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0885-4513
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-43
pubmed:dateRevised
2007-3-21
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Inhibition of P-glycoprotein and increasing of drug-sensitivity of a human carcinoma cell line (KB-A-1) by an antisense oligodeoxynucleotide-doxorubicin conjugate in vitro.
pubmed:affiliation
State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't