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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
25
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pubmed:dateCreated |
2004-6-23
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pubmed:abstractText |
Rabies virus (RV) nucleoprotein (N) tightly encapsidates the genomic and antigenomic RNA of RV to form the viral ribonucleoprotein (RNP) complex. Antigens, such as N, presented in a highly organized structure are sufficient and even desirable to activate B cells to proliferate and produce antibodies. In addition to activating B cells to proliferate, it has been shown that RV N in the RNP complex induces potent T helper cell responses resulting in long-lasting and strong humoral immune responses against RV. The possibility to systematically incorporate foreign genes into the genome of RV and produce a recombinant virus allows us to examine whether the immunogenicity of foreign antigens can be enhanced by incorporation into the RV RNP structure. To test this hypothesis we constructed a recombinant RV expressing a RV N-GFP fusion protein. The chimeric N-GFP fusion protein was efficiently expressed and incorporated into RV RNP and virions. Moreover, the recombinant RNP induces a strong humoral immune response against GFP in mice. In contrast, mice inoculated with GFP alone or a combination of wild-type RV RNPs and GFP did not trigger any GFP-specific humoral responses using the same immunization schedule. These data indicate the usefulness of RV-based vectors as killed vaccines against other infectious diseases.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-10706640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-11287595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-11878905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-11886247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-12239288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-12244598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-12477829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-12555676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-14512539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-15043205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-2139267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-2462742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-3407152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-3480536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-4196634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-6185960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-6195085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-6823748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-7513919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-7514684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-7520465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-7525709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-7542855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-7971982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-8248784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-8566036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-8692989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-8810996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-8991286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-9298897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-9689105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15197258-9847327
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
101
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9405-10
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15197258-Animals,
pubmed-meshheading:15197258-Antigens, Viral,
pubmed-meshheading:15197258-Base Sequence,
pubmed-meshheading:15197258-CD4-Positive T-Lymphocytes,
pubmed-meshheading:15197258-DNA Primers,
pubmed-meshheading:15197258-Female,
pubmed-meshheading:15197258-Genes, Reporter,
pubmed-meshheading:15197258-Lymphocyte Depletion,
pubmed-meshheading:15197258-Mice,
pubmed-meshheading:15197258-Mice, Inbred C57BL,
pubmed-meshheading:15197258-Polymerase Chain Reaction,
pubmed-meshheading:15197258-Rabies virus,
pubmed-meshheading:15197258-Recombinant Fusion Proteins,
pubmed-meshheading:15197258-Recombination, Genetic,
pubmed-meshheading:15197258-Ribonucleoproteins,
pubmed-meshheading:15197258-Viral Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
Rabies virus nucleoprotein as a carrier for foreign antigens.
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pubmed:affiliation |
Department of Biochemistry and Molecular Pharmacology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107-5541, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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