Linked Life Data

15184421

Source:http://linkedlifedata.com/resource/pubmed/id/15184421

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-6-8
pubmed:abstractText
Detection and analysis of Babesia gibsoni infection were performed with whole-blood samples collected between July 2002 and July 2003 from 945 and 137 dogs from the Aomori and Okinawa Prefectures of Japan, respectively, by PCR and loop-mediated isothermal amplification (LAMP). On the basis of the criterion for positivity by PCR, 3.9% (37 of 945) and 10.9% (15 of 137) of the dogs had B. gibsoni DNA. All 37 positive animals from Aomori Prefecture were male Tosa dogs (Japanese mastiff). The 15 dogs from Okinawa Prefecture with positive PCR assay results were of various breeds, ages, and sexes. The 18S ribosomal DNA (18S rDNA) sequences from all samples showed 100% homology to each other and to published B. gibsoni sequences. The limits of detection of B. gibsoni parasitemia by the PCR and LAMP methods with an 18S rDNA-based primer set were 0.0005% each. A comparison of the PCR and LAMP methods with microscopic examination for the detection of B. gibsoni infections in blood samples from 945 field dogs in Aomori Prefecture and 137 field dogs in Okinawa Prefecture showed that 37 and 15 dogs, respectively, were positive by the PCR and LAMP methods and that 16 and 12 dogs, respectively, were positive by light microscopic examination. All samples found to be positive by microscopic examination were also positive by the PCR and LAMP methods. The results of the PCR and LAMP methods agreed for samples with positive results by either method. Moreover, nonspecific reactions were not observed by the LAMP method. These results suggest that the LAMP method provides a useful tool for the detection of B. gibsoni infections in dogs.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-10102180, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-10871386, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-11060094, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-11642286, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-11829262, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-12465811, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-12791888, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-12808209, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-12904344, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-12958269, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-1787120, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-7061333, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-7595923, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-7756403, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-8357932, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-9267413, http://linkedlifedata.com/resource/pubmed/commentcorrection/15184421-9519430
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2465-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop-mediated isothermal amplification method.
pubmed:affiliation
Department of Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan. ikadai@vmas.kitasato-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't