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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
32
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pubmed:dateCreated |
2004-8-2
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pubmed:abstractText |
Hepatocyte nuclear factor 6 (HNF-6) belongs to the family of One Cut transcription factors (also known as OC-1) and is essential for the development of the mouse pancreas, gall bladder, and the interhepatic bile ducts. HNF-6 binds to DNA as a monomer utilizing a single cut domain and a divergent homeodomain motif located at its C terminus. Here, we have used NMR methods to determine the solution structures of the 162 amino acid residue DNA-binding domain of the HNF-6alpha protein. The resulting overall structure of HNF-6alpha has two different distinct domains: the Cut domain and the Homeodomain connected by a long flexible linker. Our NMR structure shows that the Cut domain folds into a topology homologous to the POU DNA-binding domain, even though the sequences of these two protein families do not show homology. The DNA contact sequence of the HNF-6alpha was mapped with chemical shift perturbation methods. Our data also show that a proposed CREB-binding protein histone acetyltransferase protein-recruiting sequence, LSDLL, forms a helix and is involved in the hydrophobic core of the Cut domain. The structure implies that this sequence has to undergo structural changes when it interacts with CREB-binding protein.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 6,
http://linkedlifedata.com/resource/pubmed/chemical/Histone Acetyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Hnf1a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-6,
http://linkedlifedata.com/resource/pubmed/chemical/Onecut1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Pou3f1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
279
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
33928-36
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pubmed:dateRevised |
2009-11-24
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pubmed:meshHeading |
pubmed-meshheading:15169783-Acetyltransferases,
pubmed-meshheading:15169783-Amino Acid Sequence,
pubmed-meshheading:15169783-Animals,
pubmed-meshheading:15169783-Binding Sites,
pubmed-meshheading:15169783-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:15169783-DNA,
pubmed-meshheading:15169783-DNA-Binding Proteins,
pubmed-meshheading:15169783-Hepatocyte Nuclear Factor 1,
pubmed-meshheading:15169783-Hepatocyte Nuclear Factor 1-alpha,
pubmed-meshheading:15169783-Hepatocyte Nuclear Factor 6,
pubmed-meshheading:15169783-Histone Acetyltransferases,
pubmed-meshheading:15169783-Homeodomain Proteins,
pubmed-meshheading:15169783-Magnetic Resonance Spectroscopy,
pubmed-meshheading:15169783-Mice,
pubmed-meshheading:15169783-Models, Molecular,
pubmed-meshheading:15169783-Molecular Sequence Data,
pubmed-meshheading:15169783-Molecular Structure,
pubmed-meshheading:15169783-Nuclear Proteins,
pubmed-meshheading:15169783-Octamer Transcription Factor-6,
pubmed-meshheading:15169783-Protein Folding,
pubmed-meshheading:15169783-Sequence Homology,
pubmed-meshheading:15169783-Solutions,
pubmed-meshheading:15169783-Trans-Activators,
pubmed-meshheading:15169783-Transcription Factors
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pubmed:year |
2004
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pubmed:articleTitle |
Structure of the hepatocyte nuclear factor 6alpha and its interaction with DNA.
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pubmed:affiliation |
Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60607, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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