Source:http://linkedlifedata.com/resource/pubmed/id/15153471
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
2004-5-21
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pubmed:abstractText |
Lupus-prone (NZB x NZW)F(1) (BWF(1)) mice were made transgenic (Tg) for an anti-DNA Ab inherited either as a conventional V(H)3H9- micro H chain Tg (3H9- micro ) with or without a conventional V(kappa)8-kappa Tg, or a V(H)3H9 V(H) knock-in Tg allele (3H9R) with or without a V(kappa)4 V(kappa) knock-in Tg allele (V(kappa)4R). V(H)3H9 yields an anti-DNA Ab with most L chains including an anti-ssDNA with the V(kappa)8 Tg and an anti-dsDNA with the V(kappa)4 Tg. BWF(1) mice that inherited the conventional 3H9- micro had normal serum IgM, little to none of which was encoded by 3H9- micro, and only a small percentage of those mice had serum anti-DNA, none of which was transgene encoded. B cells expressing the conventional 3H9- micro Tg were anergic. BWF(1) mice that inherited the knock-in 3H9R Tg allele also had normal serum IgM, one-half of which was encoded by 3H9R, and produced anti-DNA encoded by the Tg allele. Most B cells expressing the knock-in 3H9R Tg also had an anergic phenotype. The results indicate that autoimmune-prone BWF(1) mice initially develop effective B cell tolerance to DNA through anergy, and anergy was sustained in 3H9- micro Tg peripheral B cells but not in 3H9R Tg B cells. B cells expressing the 3H9R knock-in Tg allele were able to achieve an activation threshold that B cells expressing the 3H9- micro conventional Tg could not. The maintenance of B cell tolerance to DNA in autoimmune-prone BWF(1) mice appears to differ from both normal mice and autoimmune-prone MRL(lpr/lpr) mice.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Antinuclear,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Heavy Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
172
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6568-77
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15153471-Animals,
pubmed-meshheading:15153471-Antibodies, Antinuclear,
pubmed-meshheading:15153471-Autoimmune Diseases,
pubmed-meshheading:15153471-B-Lymphocytes,
pubmed-meshheading:15153471-DNA,
pubmed-meshheading:15153471-Female,
pubmed-meshheading:15153471-Flow Cytometry,
pubmed-meshheading:15153471-Immune Tolerance,
pubmed-meshheading:15153471-Immunoglobulin Heavy Chains,
pubmed-meshheading:15153471-Immunoglobulin M,
pubmed-meshheading:15153471-Immunoglobulin Variable Region,
pubmed-meshheading:15153471-Longevity,
pubmed-meshheading:15153471-Male,
pubmed-meshheading:15153471-Mice,
pubmed-meshheading:15153471-Mice, Inbred BALB C,
pubmed-meshheading:15153471-Mice, Inbred NZB,
pubmed-meshheading:15153471-Mice, Transgenic,
pubmed-meshheading:15153471-Transgenes
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pubmed:year |
2004
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pubmed:articleTitle |
Tolerance to DNA in (NZB x NZW)F1 mice that inherit an anti-DNA V(H) as a conventional micro H chain transgene but not as a V(H) knock-in transgene.
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pubmed:affiliation |
Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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