Linked Life Data

15147902

Source:http://linkedlifedata.com/resource/pubmed/id/15147902

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2004-5-18
pubmed:abstractText
Here, we identify ADP-ribosylation factor (ARF)-like 7 (ARL7) as the only ARF- and ARL-family member whose mRNA-expression is induced by liver X-receptor/retinoid X-receptor agonists or cholesterol loading in human macrophages. Moreover, subcellular distribution of mutant and wild type ARL7-enhanced green fluorescent protein (EGFP) supports that ARL7 may be involved in a vesicular transport step between a perinuclear compartment and the plasma membrane. Therefore, we investigated the effect of ARL7 over-expression on the cholesterol secretory pathway. We found that expression of wild type and dominant active ARL7-EGFP stimulated the rate of apolipoprotein AI-specific cholesterol efflux 1.7- and 2.8-fold. In contrast, expression of the dominant negative form of ARL7-EGFP led to approximately 50% inhibition of cholesterol efflux. This data is consistent with a model in which ARL7 is involved in transport between a perinuclear compartment and the plasma membrane apparently linked to the ABCA1-mediated cholesterol secretion pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
566
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
241-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15147902-ADP-Ribosylation Factors, pubmed-meshheading:15147902-Apolipoprotein A-I, pubmed-meshheading:15147902-Biological Transport, pubmed-meshheading:15147902-Cholesterol, pubmed-meshheading:15147902-Gene Expression, pubmed-meshheading:15147902-Green Fluorescent Proteins, pubmed-meshheading:15147902-HeLa Cells, pubmed-meshheading:15147902-Humans, pubmed-meshheading:15147902-Luminescent Proteins, pubmed-meshheading:15147902-Macrophages, pubmed-meshheading:15147902-Monocytes, pubmed-meshheading:15147902-RNA, Messenger, pubmed-meshheading:15147902-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:15147902-Receptors, Retinoic Acid, pubmed-meshheading:15147902-Recombinant Fusion Proteins, pubmed-meshheading:15147902-Subcellular Fractions, pubmed-meshheading:15147902-Transfection
pubmed:year
2004
pubmed:articleTitle
ADP-ribosylation factor (ARF)-like 7 (ARL7) is induced by cholesterol loading and participates in apolipoprotein AI-dependent cholesterol export.
pubmed:affiliation
Institut für Arterioskleroseforschung, Westfälische Wilhelms-Universität, Domagkstr. 3, D-48149 Münster, Germany. engeltho@uni-muenster.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't