Source:http://linkedlifedata.com/resource/pubmed/id/15143137
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0003762,
umls-concept:C0015663,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0035696,
umls-concept:C0036108,
umls-concept:C0185117,
umls-concept:C0205653,
umls-concept:C0243102,
umls-concept:C0332307,
umls-concept:C0441889,
umls-concept:C0443764,
umls-concept:C0680243,
umls-concept:C1832073,
umls-concept:C2911684
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| pubmed:issue |
Pt 12
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| pubmed:dateCreated |
2004-5-14
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| pubmed:abstractText |
Through analysis of a cDNA library and third-party annotation of available database sequences, we characterized the full-length coding regions of rainbow trout (Oncorhynchus mykiss) Type I, Onmy-ARG01, and Type II, Onmy-ARG02, arginase genes. Two partial related arginase sequences, Onmy-ARG01b and Onmy-ARG02b, and a full-length zebrafish arginase coding region (Danio rerio), Dare-ARG02, are also reported. Comparison of vertebrate arginase sequences shows that both Type I and Type II genes in bony fishes contain a mitochondrial targeting N-terminal domain. This suggests that the cytosolic Type I arginase found in ureotelic vertebrates arose in the common ancestor of amphibia and mammals. Onmy-ARG01 and Onmy-ARG02 mRNA was detected in liver, kidney, gill, intestine, red muscle and heart tissues. Onmy-ARG01 was expressed at a significantly higher level relative to Onmy-ARG02 in liver and red muscle tissue. To investigate whether there was differential regulation of Onmy-ARG01 and Onmy-ARG02, juvenile trout were fasted for 6 weeks and hepatic enzyme activities and mRNA levels were compared with those of fed control fish. There was a 3-fold increase in liver arginase activity and a 2-fold increase in Onmy-ARG02 mRNA levels but no change in Onmy-ARG01 mRNA levels in fasted fish relative to fed fish. These findings indicate that both types of arginase genes are present and expressed in rainbow trout and that the pattern of expression varies between tissues. The increase in liver arginase activity after a 6-week fast is due, in part, to an increase in the expression of Onmy-ARG02 mRNA levels.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0022-0949
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
207
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2033-42
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15143137-Amino Acid Sequence,
pubmed-meshheading:15143137-Analysis of Variance,
pubmed-meshheading:15143137-Animals,
pubmed-meshheading:15143137-Arginase,
pubmed-meshheading:15143137-Base Sequence,
pubmed-meshheading:15143137-Blotting, Northern,
pubmed-meshheading:15143137-Databases, Genetic,
pubmed-meshheading:15143137-Expressed Sequence Tags,
pubmed-meshheading:15143137-Food Deprivation,
pubmed-meshheading:15143137-Gene Expression,
pubmed-meshheading:15143137-Gene Library,
pubmed-meshheading:15143137-Likelihood Functions,
pubmed-meshheading:15143137-Liver,
pubmed-meshheading:15143137-Models, Genetic,
pubmed-meshheading:15143137-Molecular Sequence Data,
pubmed-meshheading:15143137-Muscle, Skeletal,
pubmed-meshheading:15143137-Oncorhynchus mykiss,
pubmed-meshheading:15143137-Phylogeny,
pubmed-meshheading:15143137-RNA, Messenger,
pubmed-meshheading:15143137-Sequence Alignment,
pubmed-meshheading:15143137-Sequence Analysis, DNA
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| pubmed:year |
2004
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| pubmed:articleTitle |
Dogmas and controversies in the handling of nitrogenous wastes: expression of arginase Type I and II genes in rainbow trout: influence of fasting on liver enzyme activity and mRNA levels in juveniles.
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| pubmed:affiliation |
Department of Zoology, University of Guelph, Guelph, ON, Canada N1G 2W1. patwrigh@uoguelph.ca
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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