|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2004-4-27
|
|
pubmed:abstractText |
We describe a method to consistently prepare human islets for transplantation. By combining a simple collagenase digestion method and a density gradient purification system, we were able to obtain successful isolations (>/=200,000 islet equivalents, >/=50% purity) in 69% of processed glands. No reagent of animal source was used. Isolated islets were morphologically well maintained and functionally competent, with sterility confirmed in 97% of cases. Two patients were transplanted with islets prepared by this method; graft function was demonstrated for a few months. Improved simplicity and consistency, together with adequate quality of the preparations, are the main features of this isolation method.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0041-1345
|
|
pubmed:author |
pubmed-author:BoggiUU,
pubmed-author:BuglianiMM,
pubmed-author:CampaMM,
pubmed-author:CampatelliAA,
pubmed-author:CorsiniVV,
pubmed-author:Del ChiaroMM,
pubmed-author:Del GuerraSS,
pubmed-author:Del PratoSS,
pubmed-author:Di CandioFF,
pubmed-author:FilipponiFF,
pubmed-author:LupiRR,
pubmed-author:MarchettiPP,
pubmed-author:MarselliLL,
pubmed-author:MoscaFF,
pubmed-author:SbranaSS,
pubmed-author:SignoriSS,
pubmed-author:TorriSS,
pubmed-author:VistoliFF
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
36
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
605-6
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:15110608-Adult,
pubmed-meshheading:15110608-Cell Separation,
pubmed-meshheading:15110608-Graft Survival,
pubmed-meshheading:15110608-Humans,
pubmed-meshheading:15110608-Islets of Langerhans,
pubmed-meshheading:15110608-Islets of Langerhans Transplantation,
pubmed-meshheading:15110608-Middle Aged,
pubmed-meshheading:15110608-Organ Preservation,
pubmed-meshheading:15110608-Tissue Donors,
pubmed-meshheading:15110608-Tissue and Organ Harvesting,
pubmed-meshheading:15110608-Treatment Outcome
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
An alternative and simple method to consistently prepare viable isolated human islets for clinical transplantation.
|
|
pubmed:affiliation |
Department of Endocrinology and Metabolism, Metabolic Unit, University of Pisa, Pisa, Italy.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
