Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2004-5-3
pubmed:databankReference
pubmed:abstractText
The molecular mechanisms underlying the formation of carriers trafficking from the Golgi complex to the cell surface are still ill-defined; nevertheless, the involvement of a lipid-based machinery is well established. This includes phosphatidylinositol 4-phosphate (PtdIns(4)P), the precursor for phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)). In yeast, PtdIns(4)P exerts a direct role, however, its mechanism of action and its targets in mammalian cells remain uncharacterized. We have identified two effectors of PtdIns(4)P, the four-phosphate-adaptor protein 1 and 2 (FAPP1 and FAPP2). Both proteins localize to the trans-Golgi network (TGN) on nascent carriers, and interact with PtdIns(4)P and the small GTPase ADP-ribosylation factor (ARF) through their plekstrin homology (PH) domain. Displacement or knockdown of FAPPs inhibits cargo transfer to the plasma membrane. Moreover, overexpression of FAPP-PH impairs carrier fission. Therefore, FAPPs are essential components of a PtdIns(4)P- and ARF-regulated machinery that controls generation of constitutive post-Golgi carriers.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1465-7392
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
393-404
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15107860-ADP-Ribosylation Factors, pubmed-meshheading:15107860-Adaptor Proteins, Signal Transducing, pubmed-meshheading:15107860-Animals, pubmed-meshheading:15107860-Biological Transport, pubmed-meshheading:15107860-COS Cells, pubmed-meshheading:15107860-Carrier Proteins, pubmed-meshheading:15107860-Cell Membrane, pubmed-meshheading:15107860-Fungal Proteins, pubmed-meshheading:15107860-Golgi Apparatus, pubmed-meshheading:15107860-Humans, pubmed-meshheading:15107860-Molecular Sequence Data, pubmed-meshheading:15107860-Phosphatidylinositol Phosphates, pubmed-meshheading:15107860-Protein Structure, Tertiary, pubmed-meshheading:15107860-RNA, Small Interfering, pubmed-meshheading:15107860-Recombinant Fusion Proteins, pubmed-meshheading:15107860-Subcellular Fractions, pubmed-meshheading:15107860-trans-Golgi Network
pubmed:year
2004
pubmed:articleTitle
FAPPs control Golgi-to-cell-surface membrane traffic by binding to ARF and PtdIns(4)P.
pubmed:affiliation
Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, via Nazionale, 66030 Santa Maria Imbaro (CH), Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't